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Cell cycle-dependent and cell cycle-independent control of transcription by the<i>Drosophila</i>E2F/RB pathway

Cell cycle-dependent and cell cycle-independent control of transcription by... Downloaded from genesdev.cshlp.org on September 27, 2021 - Published by Cold Spring Harbor Laboratory Press Cell cycle-dependent and cell cycle-independent control of transcription by the Drosophila E2F/RB pathway 1 1 2 Dessislava K. Dimova, Olivier Stevaux, Maxim V. Frolov, and Nicholas J. Dyson Massachusetts General Hospital Cancer Center, Charlestown, Massachusetts, 02129 USA To determine which E2F/RB-family members are functionally important at E2F-dependent promoters, we used RNA interference (RNAi) to selectively remove each component of the dE2F/dDP/RBF pathway, and we examined the genome-wide changes in gene expression that occur when each element is missing. The results reveal a remarkable division of labor between family members. Classic E2F targets, encoding functions needed for cell cycle progression, are expressed in cycling cells and are primarily dependent on dE2F1and RBF1for regulation. Unexpectedly, there is a second program of dE2F/RBF-dependent transcription, in which dE2F2/RBF1or dE2F2/RBF2 complexes repress gene expression in actively p roliferating cells. These new E2F target genes encode differentiation factors that are transcribed in developmentally regulated and gender-specific patterns and not in a cell cycle-regulated manner. We propose that dE2F/RBF complexes should not be viewed simply as a cell cycle regulator of transcription. Instead, dE2F/RBF-mediated repression is exerted on genes that encode http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genes & Development Unpaywall

Cell cycle-dependent and cell cycle-independent control of transcription by the<i>Drosophila</i>E2F/RB pathway

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Unpaywall
ISSN
0890-9369
DOI
10.1101/gad.1116703
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Abstract

Downloaded from genesdev.cshlp.org on September 27, 2021 - Published by Cold Spring Harbor Laboratory Press Cell cycle-dependent and cell cycle-independent control of transcription by the Drosophila E2F/RB pathway 1 1 2 Dessislava K. Dimova, Olivier Stevaux, Maxim V. Frolov, and Nicholas J. Dyson Massachusetts General Hospital Cancer Center, Charlestown, Massachusetts, 02129 USA To determine which E2F/RB-family members are functionally important at E2F-dependent promoters, we used RNA interference (RNAi) to selectively remove each component of the dE2F/dDP/RBF pathway, and we examined the genome-wide changes in gene expression that occur when each element is missing. The results reveal a remarkable division of labor between family members. Classic E2F targets, encoding functions needed for cell cycle progression, are expressed in cycling cells and are primarily dependent on dE2F1and RBF1for regulation. Unexpectedly, there is a second program of dE2F/RBF-dependent transcription, in which dE2F2/RBF1or dE2F2/RBF2 complexes repress gene expression in actively p roliferating cells. These new E2F target genes encode differentiation factors that are transcribed in developmentally regulated and gender-specific patterns and not in a cell cycle-regulated manner. We propose that dE2F/RBF complexes should not be viewed simply as a cell cycle regulator of transcription. Instead, dE2F/RBF-mediated repression is exerted on genes that encode

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Genes & Development – Unpaywall

Published: Sep 15, 2003

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