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VIABILITY AND SURVIVAL OF MOUSE EMBRYOS FOLLOWING PARENTAL EXPOSURE TO HIGH TEMPERATURE

VIABILITY AND SURVIVAL OF MOUSE EMBRYOS FOLLOWING PARENTAL EXPOSURE TO HIGH TEMPERATURE Summary.Albino ICR mice, 384 females and 48 males, were allocated to control or stress treatments. For the latter, animals were exposed to 34·5° C and 65% relative humidity for a period of 24 hr. The treatment of males commenced 48 hr before a 6-day mating schedule and of females, at 16.00 hours on the day a copulatory plug was detected. The females were killed 54 hr or 10 days after a plug had been detected. The developmental stages of embryos recovered at 54 hr were recorded and four-cell and eight-cell ova were incubated in [3H]uridine and subjected to autoradiography. Females killed at Day 10 of gestation gave estimates of pre- and postimplantation embryonic mortality.A significant increase in rectal temperatures (approximately 2° C) following treatment was taken as indicating stress had occurred. Extensive developmental retardation and/or arrest among embryos recovered from stressed females at 54 hr resulted in an increase in the proportion of two-, three- and four-cell embryos. This retardation was coincident with fewer blastomeres of four-cell (P<0·01) and eight-cell embryos (P<0·05) being able to incorporate [3H]uridine in comparison to those from control females. After male stress, developmental retardation was evident as an accumulation (P<0·05) of four-cell embryos but only the eight-cell embryos exhibited a reduction (P<0·05) in the number able to incorporate [3H]uridine. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Reproduction Bioscientifica

VIABILITY AND SURVIVAL OF MOUSE EMBRYOS FOLLOWING PARENTAL EXPOSURE TO HIGH TEMPERATURE

BELLVÉ, A. R.
Reproduction , Volume 30 (1) – Jul 1, 1972
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References (12)

Publisher
Bioscientifica
Copyright
Copyright © 1972 The Authors. All Rights Reserved.
ISSN
1470-1626
eISSN
1741-7899
DOI
10.1530/jrf.0.0300071
Publisher site
See Article on Publisher Site

Abstract

Summary.Albino ICR mice, 384 females and 48 males, were allocated to control or stress treatments. For the latter, animals were exposed to 34·5° C and 65% relative humidity for a period of 24 hr. The treatment of males commenced 48 hr before a 6-day mating schedule and of females, at 16.00 hours on the day a copulatory plug was detected. The females were killed 54 hr or 10 days after a plug had been detected. The developmental stages of embryos recovered at 54 hr were recorded and four-cell and eight-cell ova were incubated in [3H]uridine and subjected to autoradiography. Females killed at Day 10 of gestation gave estimates of pre- and postimplantation embryonic mortality.A significant increase in rectal temperatures (approximately 2° C) following treatment was taken as indicating stress had occurred. Extensive developmental retardation and/or arrest among embryos recovered from stressed females at 54 hr resulted in an increase in the proportion of two-, three- and four-cell embryos. This retardation was coincident with fewer blastomeres of four-cell (P<0·01) and eight-cell embryos (P<0·05) being able to incorporate [3H]uridine in comparison to those from control females. After male stress, developmental retardation was evident as an accumulation (P<0·05) of four-cell embryos but only the eight-cell embryos exhibited a reduction (P<0·05) in the number able to incorporate [3H]uridine.

Journal

Reproduction – Bioscientifica

Published: Jul 1, 1972

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