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Selective expression of Cre recombinase in skeletal muscle fibers

Selective expression of Cre recombinase in skeletal muscle fibers Gerald W. M. Bothe,* Jeffrey A. Haspel, Cynthia L. Smith, Heidi H. Wiener and Steven J. Burden Skirball Institute for Molecular Medicine, New York University Medical Center, New York, New York It is often difficult to study the function of genes that are widely expressed, because their critical function in one tissue can complicate or preclude study of their role in other tissues. Several lines of evidence support the idea that neuregulin (NRG) may be a synaptic signal that activates expression of certain genes in muscle nuclei positioned near the synaptic site (Burden, 1998). Since mice lacking NRG, or its receptors (ErbB2 or ErbB4) die, owing to a failure of cardiac differentiation (Lee et al., 1995; Gasmann et al., 1995; Meyer and Birchmeier, 1995), four days prior to neuromuscular synapse formation, it remains unclear whether NRG-mediated signaling is indeed required for synapse-specific gene expression. To determine the role of NRG-mediated signaling in neuromuscular synapse formation and to circumvent the problems associated with the early requirement for NRG-mediated signaling in early development, we sought to inactivate erbB genes selectively in skeletal muscle cells, using the Cre/loxP recombination system (Gu et al., 1994). These experiments require producing mice that selectively http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genesis: the Journal of Genetics and Development Wiley

Selective expression of Cre recombinase in skeletal muscle fibers

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References (10)

Publisher
Wiley
Copyright
Copyright © 2000 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1526-954X
eISSN
1526-968X
DOI
10.1002/(SICI)1526-968X(200002)26:2<165::AID-GENE22>3.0.CO;2-F
Publisher site
See Article on Publisher Site

Abstract

Gerald W. M. Bothe,* Jeffrey A. Haspel, Cynthia L. Smith, Heidi H. Wiener and Steven J. Burden Skirball Institute for Molecular Medicine, New York University Medical Center, New York, New York It is often difficult to study the function of genes that are widely expressed, because their critical function in one tissue can complicate or preclude study of their role in other tissues. Several lines of evidence support the idea that neuregulin (NRG) may be a synaptic signal that activates expression of certain genes in muscle nuclei positioned near the synaptic site (Burden, 1998). Since mice lacking NRG, or its receptors (ErbB2 or ErbB4) die, owing to a failure of cardiac differentiation (Lee et al., 1995; Gasmann et al., 1995; Meyer and Birchmeier, 1995), four days prior to neuromuscular synapse formation, it remains unclear whether NRG-mediated signaling is indeed required for synapse-specific gene expression. To determine the role of NRG-mediated signaling in neuromuscular synapse formation and to circumvent the problems associated with the early requirement for NRG-mediated signaling in early development, we sought to inactivate erbB genes selectively in skeletal muscle cells, using the Cre/loxP recombination system (Gu et al., 1994). These experiments require producing mice that selectively

Journal

Genesis: the Journal of Genetics and Development – Wiley

Published: Feb 1, 2000

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