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Selection of Tumor-Host Cell Hybrids From Polyoma Virus- and Methylcholanthrene-Induced Sarcomas

Selection of Tumor-Host Cell Hybrids From Polyoma Virus- and Methylcholanthrene-Induced Sarcomas Summary Tumor-host cell hybrids were isolated in vitro from 2 polyoma-induced ascites sarcomas and 1 methylcholanthrene-induced sarcoma by selection of surface-adherent cell colonies. The loosely attached tumor cells were removed by shaking or mild trypsinization. A difference in the sensitivity of the tumor cells and the tumor-host cell hybrids to Colce-mid treatment provided an additional tool for selection. The hybrids were identified by chromosomal and antigenic markers. Several hybrid lines derived from the fusions between tumor cells and host cells in vivo were tested for their ability to grow progressively in F1 hybrid hosts; they were all tumorigenic. The chromosomal constitution of the tumors approximated that of the corresponding line in vitro. 2 Supported by Public Health Service research grant No.IRO1 CA14054-01 from the National Cancer Institute, and by grants from the Swedish Cancer Society, the Jane Coffin Childs Memorial Fund for Cancer Research, the Damon Runyon Memorial Fund, and the Cancer Research Campaign. This content is only available as a PDF. Author notes 5 We gratefully acknowledge the expert technical assistance of Mrs. Gunilla Isacson, Miss Bodil Lidin, and Mrs. Maj-Lis Solberg. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png JNCI: Journal of the National Cancer Institute Oxford University Press

Selection of Tumor-Host Cell Hybrids From Polyoma Virus- and Methylcholanthrene-Induced Sarcomas

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Publisher
Oxford University Press
ISSN
0027-8874
eISSN
1460-2105
DOI
10.1093/jnci/51.6.1865
Publisher site
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Abstract

Summary Tumor-host cell hybrids were isolated in vitro from 2 polyoma-induced ascites sarcomas and 1 methylcholanthrene-induced sarcoma by selection of surface-adherent cell colonies. The loosely attached tumor cells were removed by shaking or mild trypsinization. A difference in the sensitivity of the tumor cells and the tumor-host cell hybrids to Colce-mid treatment provided an additional tool for selection. The hybrids were identified by chromosomal and antigenic markers. Several hybrid lines derived from the fusions between tumor cells and host cells in vivo were tested for their ability to grow progressively in F1 hybrid hosts; they were all tumorigenic. The chromosomal constitution of the tumors approximated that of the corresponding line in vitro. 2 Supported by Public Health Service research grant No.IRO1 CA14054-01 from the National Cancer Institute, and by grants from the Swedish Cancer Society, the Jane Coffin Childs Memorial Fund for Cancer Research, the Damon Runyon Memorial Fund, and the Cancer Research Campaign. This content is only available as a PDF. Author notes 5 We gratefully acknowledge the expert technical assistance of Mrs. Gunilla Isacson, Miss Bodil Lidin, and Mrs. Maj-Lis Solberg.

Journal

JNCI: Journal of the National Cancer Institute – Oxford University Press

Published: Dec 1, 1973

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