Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/the-american-physiological-society/single-cell-rna-sequencing-of-mouse-left-ventricle-reveals-cellular-Vzd92Iz1xb
References (40)
-
S Yamada, S Nomura (2020)
Review of single-cell RNA sequencing in the heart, 21
-
HB Moeller, ET Olesen, RA Fenton (2011)
Regulation of the water channel aquaporin-2 by posttranslational modification, 300
-
M Litviňuková, C Talavera-López, H Maatz, D Reichart, CL Worth, EL Lindberg, M Kanda, K Polanski, M Heinig, M Lee, ER Nadelmann, K Roberts, L Tuck, ES Fasouli, DM DeLaughter, B McDonough, H Wakimoto, JM Gorham, S Samari, KT Mahbubani, K Saeb-Parsy, G Patone, JJ Boyle, H Zhang, H Zhang, A Viveiros, GY Oudit, OA Bayraktar, JG Seidman, CE Seidman, M Noseda, N Hubner, SA Teichmann (2020)
Cells of the adult human heart, 588
-
S Zhang, M Hong, Y Gao (2012)
Effect of oxidized LDL on the expression of connexins in cultured human umbilical-vein endothelial cells, 36
-
HJ Choi, SS Rho, DH Choi, YG Kwon (2018)
LDB2 regulates the expression of DLL4 through the formation of oligomeric complexes in endothelial cells, 51
-
N Farbehi, R Patrick, A Dorison, M Xaymardan, V Janbandhu, K Wystub-Lis, JW Ho, RE Nordon, RP Harvey (2019)
Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury, 8
-
KL Ford, M Anwar, R Heys, EM Ahmed, M Caputo, L Game, BC Reeves, PP Punjabi, GD Angelini, E Petretto, C Emanueli (2019)
Optimisation of laboratory methods for whole transcriptomic RNA analyses in human left ventricular biopsies and blood samples of clinical relevance, 14
-
HY Ren, A Khera, JA de Lemos, CR Ayers, A Rohatgi (2017)
Soluble endothelial cell-selective adhesion molecule and incident cardiovascular events in a multiethnic population, 191
-
J Chen, MC Zhong, H Guo, D Davidson, S Mishel, Y Lu, I Rhee, LA Pérez-Quintero, S Zhang, ME Cruz-Munoz, N Wu, DC Vinh, M Sinha, V Calderon, CA Lowell, JS Danska, A Veillette (2017)
SLAMF7 is critical for phagocytosis of haematopoietic tumour cells via Mac-1 integrin, 544
-
Z Zhang, P Xu, Z Xie, F Shen, N Chen, L Yu, R He (2017)
Downregulation of AQP2 in the anterior vaginal wall is associated with the pathogenesis of female stress urinary incontinence, 16
-
A Butler, P Hoffman, P Smibert, E Papalexi, R Satija (2018)
Integrating single-cell transcriptomic data across different conditions, technologies, and species, 36
-
N Chopra, BC Knollmann (2013)
Triadin regulates cardiac muscle couplon structure and microdomain Ca2+ signalling: a path towards ventricular arrhythmias, 98
-
S Dalal, Q Zha, M Singh, K Singh (2016)
Osteopontin-stimulated apoptosis in cardiac myocytes involves oxidative stress and mitochondrial death pathway: role of a pro-apoptotic protein BIK, 418
-
X Han, R Wang, Y Zhou, L Fei, H Sun, S Lai, A Saadatpour, Z Zhou, H Chen, F Ye, D Huang, Y Xu, W Huang, M Jiang, X Jiang, J Mao, Y Chen, C Lu, J Xie, Q Fang, Y Wang, R Yue, T Li, H Huang, SH Orkin, GC Yuan, M Chen, G Guo (2018)
Mapping the mouse cell atlas by microwell-seq, 172
-
JH Lee, C Gao, G Peng, C Greer, S Ren, Y Wang, X Xiao (2011)
Analysis of transcriptome complexity through RNA sequencing in normal and failing murine hearts, 109
-
BJ Vorderwülbecke, J Maroski, K Fiedorowicz, L Da Silva-Azevedo, A Marki, AR Pries, A Zakrzewicz (2012)
Regulation of endothelial connexin40 expression by shear stress, 302
-
MA McLellan, DA Skelly, MSI Dona, GT Squiers, GE Farrugia, TL Gaynor, CD Cohen, R Pandey, H Diep, A Vinh, NA Rosenthal, AR Pinto (2020)
High-resolution transcriptomic profiling of the heart during chronic stress reveals cellular drivers of cardiac fibrosis and hypertrophy, 142
-
Y Hu, SJ Matkovich, PA Hecker, Y Zhang, JR Edwards, GW Dorn (2012)
Epitranscriptional orchestration of genetic reprogramming is an emergent property of stress-regulated cardiac microRNAs, 109
-
DA Skelly, GT Squiers, MA McLellan, MT Bolisetty, P Robson, NA Rosenthal, AR Pinto (2018)
Single-cell transcriptional profiling reveals cellular diversity and intercommunication in the mouse heart, 22
-
C Zeltz, D Gullberg (2016)
The integrin-collagen connection – a glue for tissue repair?, 129
-
D Jia, P Bai, N Wan, J Liu, Q Zhu, Y He, G Chen, J Wang, H Chen, C Wang, A Lyu, M Lazarus, Y Su, Y Urade, Y Yu, J Zhang, Y Shen (2020)
Niacin attenuates pulmonary hypertension through H-PGDS in macrophages, 127
-
M Clément, G Basatemur, L Masters, L Baker, P Bruneval, T Iwawaki, M Kneilling, S Yamasaki, J Goodall, Z Mallat (2016)
Necrotic cell sensor Clec4e promotes a proatherogenic macrophage phenotype through activation of the unfolded protein response, 134
-
E Tanai, S Frantz (2015)
Pathophysiology of heart failure, 6
-
P Xu, X Zhang, Q Liu, Y Xie, X Shi, J Chen, Y Li, H Guo, R Sun, Y Hong, X Liu, G Xu (2019)
Microglial TREM-1 receptor mediates neuroinflammatory injury via interaction with SYK in experimental ischemic stroke, 10
-
S Fukada, Y Yamamoto, M Segawa, K Sakamoto, M Nakajima, M Sato, D Morikawa, A Uezumi, Y Miyagoe-Suzuki, S Takeda, K Tsujikawa, H Yamamoto (2008)
CD90-positive cells, an additional cell population, produce laminin α2 upon transplantation to dy(3k)/dy(3k) mice, 314
-
S Muppala, R Xiao, I Krukovets, D Verbovetsky, R Yendamuri, N Habib, P Raman, E Plow, O Stenina-Adognravi (2017)
Thrombospondin-4 mediates TGF-β-induced angiogenesis, 36
-
RJ Schwartz, EN Olson (1999)
Building the heart piece by piece: modularity of cis-elements regulating Nkx2-5 transcription, 126
-
CW Wong, T Christen, I Roth, CE Chadjichristos, JP Derouette, BF Foglia, M Chanson, DA Goodenough, BR Kwak (2006)
Connexin37 protects against atherosclerosis by regulating monocyte adhesion, 12
-
Y Wang, F Yao, L Wang, Z Li, Z Ren, D Li, M Zhang, L Han, SQ Wang, B Zhou, L Wang (2020)
Single-cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation, 11
-
K Palumbo-Zerr, A Soare, P Zerr, A Liebl, R Mancuso, M Tomcik, B Sumova, C Dees, CW Chen, T Wohlfahrt, T Mallano, A Distler, A Ramming, K Gelse, C Mihai, O Distler, G Schett, JH Distler (2017)
Composition of TWIST1 dimers regulates fibroblast activation and tissue fibrosis, 76
-
HK Song, SE Hong, T Kim, DH Kim (2012)
Deep RNA sequencing reveals novel cardiac transcriptomic signatures for physiological and pathological hypertrophy, 7
-
D Lambrechts, E Wauters, B Boeckx, S Aibar, D Nittner, O Burton, A Bassez, H Decaluwé, A Pircher, K Van den Eynde, B Weynand, E Verbeken, P De Leyn, A Liston, J Vansteenkiste, P Carmeliet, S Aerts, B Thienpont (2018)
Phenotype molding of stromal cells in the lung tumor microenvironment, 24
-
JG Dumesnil, RM Shoucri (1982)
Effect of the geometry of the left ventricle on the calculation of ejection fraction, 65
-
AA Derda, CC Woo, T Wongsurawat, M Richards, CN Lee, T Kofidis, VA Kuznetsov, VA Sorokin (2018)
Gene expression profile analysis of aortic vascular smooth muscle cells reveals upregulation of cadherin genes in myocardial infarction patients, 50
-
L Jérémie, B Amir, D Marc, G Sébastien (2015)
The triggering receptor expressed on myeloid cells-1: a new player during acute myocardial infarction, 100
-
MM Markiewski, SK Vadrevu, SK Sharma, NK Chintala, S Ghouse, JH Cho, DP Fairlie, Y Paterson, A Astrinidis, M Karbowniczek (2017)
The ribosomal protein S19 suppresses antitumor immune responses via the complement C5a receptor 1, 198
-
SC Lütken, J Frøkiær, S Nielsen (2015)
AVP-induced increase in AQP2 and p-AQP2 is blunted in heart failure during cardiac remodeling and is associated with decreased AT1R abundance in rat kidney, 10
-
EZ Macosko, A Basu, R Satija, J Nemesh, K Shekhar, M Goldman, I Tirosh, AR Bialas, N Kamitaki, EM Martersteck, JJ Trombetta, DA Weitz, JR Sanes, AK Shalek, A Regev, SA McCarroll (2015)
Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets, 161
-
M Park, A Kulkarni, A Beatty, P Ganz, M Regan, E Vittinghoff, M Whooley (2015)
Soluble endothelial cell selective adhesion molecule and cardiovascular outcomes in patients with stable coronary disease: a report from the Heart and Soul Study, 243
-
J Liao, X Lu, X Shao, L Zhu, X Fan (2021)
Uncovering an organ's molecular architecture at single-cell resolution by spatially resolved transcriptomics, 39
- ISSN
- 1094-8341
- eISSN
- 1531-2267
- DOI
- 10.1152/physiolgenomics.00016.2021
- Publisher site
- See Article on Publisher Site
Abstract
Previous studies have revealed the diversity of the whole cardiac cellulome but not refined the left ventricle, which was essential for finding therapeutic targets. Here, we characterized single-cell transcriptional profiles of the mouse left ventricular cellular landscape using single-cell RNA sequencing (10×Genomics). Detailed t-Distributed Stochastic Neighbor Embedding (tSNE) analysis revealed the cell types of left ventricle with gene markers. Left ventricular cellulome contained cardiomyocytes highly expressed Trdn, endothelial cells highly expressed Pcdh17, fibroblast highly expressed Lama2 and macrophages highly expressed Hpgds, also proved by in situ hybridization. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analysis (ListHits>2, p<0.05) were employed with the DAVID database to investigate subtypes of each cell type with the underlying functions of differentially expressed genes (DEGs). Endothelial cells included five subtypes, fibroblasts comprised of seven subtypes and macrophages contained eleven subtypes. The key representative DEGs (p<0.001) were Gja4 and Gja5 in cluster 3 of endothelial cells, Aqp2 and Thbs4 in cluster 2 of fibroblasts, as well as Clec4e and Trem-1 in in cluster 3 of marcophages perhaps involved in the occur of atherosclerosis, heart failure and acute myocardial infarction proved by literature review. We also revealed extensive networks of intercellular communication in left ventricle. We suggested possible therapeutic targets for cardiovascular disease and autocrine and paracrine signaling underpins left ventricular homeostasis. This study provided new insights into the structure and function of the mammalian left ventricular cellulome and offers an important resource that will stimulate studies in cardiovascular research.
Journal
Physiological Genomics – The American Physiological Society
Published: Jan 1, 2022