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Specificity of late-night salivary cortisol measured by automated electrochemiluminescence immunoassay for Cushing’s disease in an obese population

Specificity of late-night salivary cortisol measured by automated electrochemiluminescence... Purpose Data about the specificity of late-night salivary cortisol (LNSC) in obese subjects are still conflicting. Therefore, with this study, we aimed to evaluate the specificity of LNSC measurement in an obese cohort with or without type 2 diabetes mellitus (T2DM) using an automated electrochemiluminescence immunoassay (ECLIA). Methods A total number of 157 patients involving 40 healthy subjects (HS) with BMI < 25 kg/m , 83 obese subjects (OS) with BMI ≥ 35  kg/m , and 34 histopathologically proven Cushing’s disease (CD) were included. All patients underwent LNSC testing. Salivary cortisol was measured at 11 p.m. for all groups using an ECLIA. Reference range was established using values of LNSCs of HS and ROC curves were used to determine diagnostic cutoffs. Results In the HS group, mean LNSC was 4.7 nmol/l (SD ± 3.1), while the OS group had a mean value of 10.9 nmol/l (SD ± 7.5) and the CD group of 19.9 nmol/l (SD ± 15.4). All groups differed significantly ( p < 0.001). The ROC analysis of CD against HS alone showed a sensitivity of 85.3% and a specificity of 87.5% with a cut-off value of 8.3 nmol/l. The ROC analysis between OS and CD showed a maximum sensitivity of 67.6% and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Endocrinological Investigation Springer Journals

Specificity of late-night salivary cortisol measured by automated electrochemiluminescence immunoassay for Cushing’s disease in an obese population

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References (25)

Publisher
Springer Journals
Copyright
Copyright © 2018 by Italian Society of Endocrinology (SIE)
Subject
Medicine & Public Health; Medicine/Public Health, general; Endocrinology; Metabolic Diseases
eISSN
1720-8386
DOI
10.1007/s40618-018-0870-8
Publisher site
See Article on Publisher Site

Abstract

Purpose Data about the specificity of late-night salivary cortisol (LNSC) in obese subjects are still conflicting. Therefore, with this study, we aimed to evaluate the specificity of LNSC measurement in an obese cohort with or without type 2 diabetes mellitus (T2DM) using an automated electrochemiluminescence immunoassay (ECLIA). Methods A total number of 157 patients involving 40 healthy subjects (HS) with BMI < 25 kg/m , 83 obese subjects (OS) with BMI ≥ 35  kg/m , and 34 histopathologically proven Cushing’s disease (CD) were included. All patients underwent LNSC testing. Salivary cortisol was measured at 11 p.m. for all groups using an ECLIA. Reference range was established using values of LNSCs of HS and ROC curves were used to determine diagnostic cutoffs. Results In the HS group, mean LNSC was 4.7 nmol/l (SD ± 3.1), while the OS group had a mean value of 10.9 nmol/l (SD ± 7.5) and the CD group of 19.9 nmol/l (SD ± 15.4). All groups differed significantly ( p < 0.001). The ROC analysis of CD against HS alone showed a sensitivity of 85.3% and a specificity of 87.5% with a cut-off value of 8.3 nmol/l. The ROC analysis between OS and CD showed a maximum sensitivity of 67.6% and

Journal

Journal of Endocrinological Investigation – Springer Journals

Published: Mar 17, 2018

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