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Role for a Xenopus Orc2-related protein in controlling DNA replication

Role for a Xenopus Orc2-related protein in controlling DNA replication THE six-subunit origin recognition complex (ORC) is essential for the initiation of DNA replication at specific origins in the budding yeast Saccharomyces cerevisiae 1–9. An important issue is whether DNA replication in higher eukaryotes, in which the characteristics of replication origins are poorly defined10, occurs by an ORC-dependent mechanism. We have identified a Xenopus laevis Orel-related protein (XORC2) by its ability to rescue a mitotic-catastrophe mutant of the fission yeast Schizosaccharomyces pombe. We show that immunodepletion of XORC2 from Xenopus egg extracts11–13 abolishes the replication of chromosomal DNA but not elongation synthesis on a single-stranded DNA template. Indirect immunofluorescence indicates that XORC2 binds to chromatin well before the commencement of DNA synthesis, and even under conditions that prevent the association of replication licensing factor(s) with the DNA. These findings suggest that Orc2 plays an important role at an early step of chromosomal replication in animal cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Springer Journals

Role for a Xenopus Orc2-related protein in controlling DNA replication

Carpenter, Phillip B.; Mueller, Paul R.; Dunphy, William G.
Nature , Volume 379 (6563) – Jan 25, 1996
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References (30)

Publisher
Springer Journals
Copyright
Copyright © 1996 by Nature Publishing Group
Subject
Science, Humanities and Social Sciences, multidisciplinary; Science, Humanities and Social Sciences, multidisciplinary; Science, multidisciplinary
ISSN
0028-0836
eISSN
1476-4687
DOI
10.1038/379357a0
Publisher site
See Article on Publisher Site

Abstract

THE six-subunit origin recognition complex (ORC) is essential for the initiation of DNA replication at specific origins in the budding yeast Saccharomyces cerevisiae 1–9. An important issue is whether DNA replication in higher eukaryotes, in which the characteristics of replication origins are poorly defined10, occurs by an ORC-dependent mechanism. We have identified a Xenopus laevis Orel-related protein (XORC2) by its ability to rescue a mitotic-catastrophe mutant of the fission yeast Schizosaccharomyces pombe. We show that immunodepletion of XORC2 from Xenopus egg extracts11–13 abolishes the replication of chromosomal DNA but not elongation synthesis on a single-stranded DNA template. Indirect immunofluorescence indicates that XORC2 binds to chromatin well before the commencement of DNA synthesis, and even under conditions that prevent the association of replication licensing factor(s) with the DNA. These findings suggest that Orc2 plays an important role at an early step of chromosomal replication in animal cells.

Journal

Nature – Springer Journals

Published: Jan 25, 1996

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