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- Publisher
- Springer Journals
- Copyright
- Copyright © 1994 by Springer-Verlag
- Subject
- Biomedicine; Human Genetics; Molecular Medicine; Gene Function; Metabolic Diseases
- ISSN
- 0340-6717
- eISSN
- 1432-1203
- DOI
- 10.1007/BF00211013
- Publisher site
- See Article on Publisher Site
Abstract
The retinoblastoma susceptibility (RB1) gene contains an unmethylated CpG-rich island at its 5′ end. Using methylation-sensitive restriction enzymes, we have investigated the methylation status of this island in 21 sporadic unilateral retinoblastomas and 30 hereditary retinoblastomas. Three sporadic unilateral tumors were found to have hypermethylated RB1 alleles. In two tumors, the paternal allele was methylated, whereas the maternal allele had been lost. Cultured cells from one of these tumors were studied by the reverse transcription polymerase chain reaction and found to have a reduced level of RB1 mRNA. The third tumor had retained constitutional heterozygosity, and the paternal allele was specifically methylated. The combined data from previously published reports and from this study show that hypermethylation of the RB1 gene occurs in 13% of sporadic unilateral tumors and may reduce gene activity.
Journal
Human Genetics – Springer Journals
Published: Jul 17, 2004