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Adhesion behavior of monocytes, macrophages, and foreign body giant cells on poly (N‐isopropylacrylamide) temperature‐responsive surfaces

Adhesion behavior of monocytes, macrophages, and foreign body giant cells on poly... Monocyte and macrophage adhesion and foreign body giant cell (FBGC) formation has been observed on surfaces with a wide range of properties. In this study we have utilized novel, temperature‐responsive surfaces (TRS) with dynamic surface properties to investigate inflammatory cell adhesion behavior. With temperature changes, grafted chains of poly‐N‐isopropylacrylamide pass through their lower critical solution temperature (LCST) and can either extend (hydrate), creating a hydrophilic surface at 20°C, or contract (dehydrate), creating a hydrophobic surface at 37°C. Isolated human monocytes and monocyte‐derived macrophages were able to adhere, spread, and form FBGC on the hydrophobic surface. Decreasing the temperature below the lower critical solution temperature induced a change in the surface wettability, creating a hydrophilic surface, that induced a differential detachment of adherent cells that decreased with time, ranging from 98% after 2 h of culture to 30% at day 10. These detached cells remained viable, and were recultured onto TCPS for 3, 7, and 10 days. These novel surfaces allow investigation of the adhesive behavior of adherent inflammatory cells in a temporal manner, and the effects of surface conformation and wettability changes on cell adhesion and detachment. © 2001 Wiley Periodicals, Inc. J Biomed Mater Res 59: 136–143, 2002 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biomedical Materials Research Part A Wiley

Adhesion behavior of monocytes, macrophages, and foreign body giant cells on poly (N‐isopropylacrylamide) temperature‐responsive surfaces

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References (38)

Publisher
Wiley
Copyright
Copyright © 2002 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1549-3296
eISSN
1552-4965
DOI
10.1002/jbm.1225
Publisher site
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Abstract

Monocyte and macrophage adhesion and foreign body giant cell (FBGC) formation has been observed on surfaces with a wide range of properties. In this study we have utilized novel, temperature‐responsive surfaces (TRS) with dynamic surface properties to investigate inflammatory cell adhesion behavior. With temperature changes, grafted chains of poly‐N‐isopropylacrylamide pass through their lower critical solution temperature (LCST) and can either extend (hydrate), creating a hydrophilic surface at 20°C, or contract (dehydrate), creating a hydrophobic surface at 37°C. Isolated human monocytes and monocyte‐derived macrophages were able to adhere, spread, and form FBGC on the hydrophobic surface. Decreasing the temperature below the lower critical solution temperature induced a change in the surface wettability, creating a hydrophilic surface, that induced a differential detachment of adherent cells that decreased with time, ranging from 98% after 2 h of culture to 30% at day 10. These detached cells remained viable, and were recultured onto TCPS for 3, 7, and 10 days. These novel surfaces allow investigation of the adhesive behavior of adherent inflammatory cells in a temporal manner, and the effects of surface conformation and wettability changes on cell adhesion and detachment. © 2001 Wiley Periodicals, Inc. J Biomed Mater Res 59: 136–143, 2002

Journal

Journal of Biomedical Materials Research Part A – Wiley

Published: Jan 1, 2002

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