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Allo‐ and self‐restricted cytotoxic T lymphocytes against a peptide library: evidence for a functionally diverse allorestricted T cell repertoire

Allo‐ and self‐restricted cytotoxic T lymphocytes against a peptide library: evidence for a... BALB/c‐derived spleen cells were depleted of cytotoxic T lymphocytes (CTL) recognizing allogeneic (H2b) and TAP‐negative cells followed by stimulation with the same cells loaded with a synthetic library binding to H2‐Kb. The resulting CTL lines were found to differ widely in peptide specificity and to exhibit an avidity towards the library as that demonstrated for syngeneic CTL. These results demonstrate that positive selection in the context of a certain MHC molecule does not seem to be required for generating high‐avidity TCR that are restricted by the same molecule. However, positive selection increases the frequency of such CTL. By raising T cell lines from a repertoire which did not undergo negative selection by the restriction element in question, it becomes possible to produce effective self‐peptide/MHC as well as nonself‐peptide/MHC‐specific CTL as tools for adoptive tumor immunotherapy. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Immunology Wiley

Allo‐ and self‐restricted cytotoxic T lymphocytes against a peptide library: evidence for a functionally diverse allorestricted T cell repertoire

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References (65)

Publisher
Wiley
Copyright
© 1998 WILEY‐VCH Verlag GmbH, Weinheim, Fed. Rep. of Germany
ISSN
0014-2980
eISSN
1521-4141
DOI
10.1002/(SICI)1521-4141(199808)28:08<2432::AID-IMMU2432>3.0.CO;2-0
pmid
9710221
Publisher site
See Article on Publisher Site

Abstract

BALB/c‐derived spleen cells were depleted of cytotoxic T lymphocytes (CTL) recognizing allogeneic (H2b) and TAP‐negative cells followed by stimulation with the same cells loaded with a synthetic library binding to H2‐Kb. The resulting CTL lines were found to differ widely in peptide specificity and to exhibit an avidity towards the library as that demonstrated for syngeneic CTL. These results demonstrate that positive selection in the context of a certain MHC molecule does not seem to be required for generating high‐avidity TCR that are restricted by the same molecule. However, positive selection increases the frequency of such CTL. By raising T cell lines from a repertoire which did not undergo negative selection by the restriction element in question, it becomes possible to produce effective self‐peptide/MHC as well as nonself‐peptide/MHC‐specific CTL as tools for adoptive tumor immunotherapy.

Journal

European Journal of Immunology – Wiley

Published: Aug 1, 1998

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