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A transgene containing lacZ inserted into the dystonia locus is expressed in neural tube

A transgene containing lacZ inserted into the dystonia locus is expressed in neural tube The site of integration of transgenes in the host genome can affect levels of expression1 and occasionally confer ectopic patterns of expression on otherwise tissue-specific genes2. We describe here a line of mice in which an hsp68-lacZ transgene is expressed in unstressed developing neural tissue and where the transgene insertion has caused a mutation of a neural tissue-specific gene, dystonia musculorum (dt). This coincidence suggests that expression of the hsp68-lacZ construct may be controlled directly by cis-acting regulatory sequences that normally control the developmental expression of the dt gene. Such constructs may serve as useful tools for identifying new tissue-specific enhancers and their associated genes3,4. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Springer Journals

A transgene containing lacZ inserted into the dystonia locus is expressed in neural tube

Kothary, R.; Clapoff, S.; Brown, A.; Campbell, R.; Peterson, A.; Rossant, J.
Nature , Volume 335 (6189) – Sep 29, 1988
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References (10)

Publisher
Springer Journals
Copyright
Copyright © 1988 by Nature Publishing Group
Subject
Science, Humanities and Social Sciences, multidisciplinary; Science, Humanities and Social Sciences, multidisciplinary; Science, multidisciplinary
ISSN
0028-0836
eISSN
1476-4687
DOI
10.1038/335435a0
Publisher site
See Article on Publisher Site

Abstract

The site of integration of transgenes in the host genome can affect levels of expression1 and occasionally confer ectopic patterns of expression on otherwise tissue-specific genes2. We describe here a line of mice in which an hsp68-lacZ transgene is expressed in unstressed developing neural tissue and where the transgene insertion has caused a mutation of a neural tissue-specific gene, dystonia musculorum (dt). This coincidence suggests that expression of the hsp68-lacZ construct may be controlled directly by cis-acting regulatory sequences that normally control the developmental expression of the dt gene. Such constructs may serve as useful tools for identifying new tissue-specific enhancers and their associated genes3,4.

Journal

Nature – Springer Journals

Published: Sep 29, 1988

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