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- Publisher
- Oxford University Press
- Copyright
- © Oxford University Press
- ISSN
- 0022-0957
- eISSN
- 1460-2431
- DOI
- 10.1093/jxb/48.8.1609
- Publisher site
- See Article on Publisher Site
Abstract
Abstract The tetraammonium salt of the K+ binding fluorescent dye benzofuran isophthalate (PBFI) was used to investigate the influence of potassium nutrition (0.1–2.1 mol m−3) on apoplastic K+ inVicia faba leaves by means of ratio imaging. As a reference the infiltration-centrifugation method was used. Both methods reflected the influence of K+ supply on apoplastic K+ concentration. The abaxial leaf side revealed significantly higher K+ concentrations (20-25 mol m−3) than the adaxial side (5–8 mol m−3). Application of CCCP led to an immediate increase in apoplastic K+ demonstrating the reliability of the PBFI method. Vicia faba, leaf, apoplast, K+, PBFI, ratio imaging, ratiometric fluorescence microscopy © Oxford University Press
Journal
Journal of Experimental Botany – Oxford University Press
Published: Aug 1, 1997