Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Synergistic induction of nitric oxide synthase type II: In vitro effect of leptin and interferon‐γ in human chondrocytes and ATDC5 chondrogenic cells

Synergistic induction of nitric oxide synthase type II: In vitro effect of leptin and... Objective To study, in vitro, the effect of leptin (OB), alone or in combination with interferon‐γ (IFNγ), on inducible nitric oxide synthase (iNOS) and NO production in human primary chondrocytes and in mouse embryonic chondrogenic ATDC5 cells. Methods Leptin receptor expression and iNOS messenger RNA expression were evaluated by reverse transcriptase–polymerase chain reaction. Then, iNOS activity was indirectly studied by measuring nitrite accumulation, using the Griess colorimetric reaction, in culture medium of human primary chondrocytes and ATDC5 cells. Results ATDC5 mouse embryonic cells expressed functional OB receptor. Alone, neither OB nor IFNγ produced nitrite accumulation in culture medium. However, costimulation with OB and IFNγ resulted in dose‐dependent up‐regulation of the expression of iNOS and NO production in human primary chondrocytes and ATDC5 cells. Production of NO was blunted by the iNOS‐specific inhibitors L‐NG‐nitroarginine methyl ester and aminoguanidine. In addition, the janus‐activated kinase 2 (JAK2)–specific inhibitor Tyrphostin AG 490 completely blocked OB + IFNγ–driven up‐regulation of iNOS and NO production. Conclusion Our data show for the first time a putative proinflammatory role of OB via iNOS induction and NO production. This occurs via activation of JAK2. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Arthritis & Rheumatism Wiley

Synergistic induction of nitric oxide synthase type II: In vitro effect of leptin and interferon‐γ in human chondrocytes and ATDC5 chondrogenic cells

Download PDF
6 pages

Loading next page...
 
/lp/wiley/synergistic-induction-of-nitric-oxide-synthase-type-ii-in-vitro-effect-RiRUkpPguG

References (17)

Publisher
Wiley
Copyright
Copyright © 2003 by the American College of Rheumatology
ISSN
0004-3591
eISSN
1529-0131
DOI
10.1002/art.10811
pmid
12571850
Publisher site
See Article on Publisher Site

Abstract

Objective To study, in vitro, the effect of leptin (OB), alone or in combination with interferon‐γ (IFNγ), on inducible nitric oxide synthase (iNOS) and NO production in human primary chondrocytes and in mouse embryonic chondrogenic ATDC5 cells. Methods Leptin receptor expression and iNOS messenger RNA expression were evaluated by reverse transcriptase–polymerase chain reaction. Then, iNOS activity was indirectly studied by measuring nitrite accumulation, using the Griess colorimetric reaction, in culture medium of human primary chondrocytes and ATDC5 cells. Results ATDC5 mouse embryonic cells expressed functional OB receptor. Alone, neither OB nor IFNγ produced nitrite accumulation in culture medium. However, costimulation with OB and IFNγ resulted in dose‐dependent up‐regulation of the expression of iNOS and NO production in human primary chondrocytes and ATDC5 cells. Production of NO was blunted by the iNOS‐specific inhibitors L‐NG‐nitroarginine methyl ester and aminoguanidine. In addition, the janus‐activated kinase 2 (JAK2)–specific inhibitor Tyrphostin AG 490 completely blocked OB + IFNγ–driven up‐regulation of iNOS and NO production. Conclusion Our data show for the first time a putative proinflammatory role of OB via iNOS induction and NO production. This occurs via activation of JAK2.

Journal

Arthritis & Rheumatism – Wiley

Published: Feb 1, 2003

There are no references for this article.