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Overexpression of the maize homeo box gene, KNOTTED-1, causes a switch from determinate to indeterminate cell fates.

Overexpression of the maize homeo box gene, KNOTTED-1, causes a switch from determinate to... Downloaded from genesdev.cshlp.org on October 23, 2021 - Published by Cold Spring Harbor Laboratory Press Overexpression of the maize homeo box gene, KNOTTED-I, causes a switch from determinate to indeterminate cell fates Neelima R. Sinha, Rosalind E. Williams, and Sarah Hake U.S. Department of Agriculture-Agricultural Research Service Plant Gene Expression Center, Albany, California 94710 USA; Department of Plant Biology, University of California, Berkeley, California 94720 USA. The KNOTTED-1 (KN1) locus of maize is defined by dominant mutations that affect leaf cell fates. Transposon tagging led to the isolation of the gene and the discovery that KN1 encodes a homeo domain. Immunolocalization studies showed that in wild-type maize plants, KN1 protein is present in nuclei of apical meristems and immature shoot axes but is down-regulated as lateral organs, such as leaves, are initiated. The protein is not immunohistochemically detectable in wild-type leaves at any stage. In developing leaves of plants carrying the dominant Knl mutation, temporally and spatially restricted ectopic expression of KN1 causes the mutant phenotype. To better understand the function of KN1 in plant development, we sought to determine the phenotype of plants in which KN1 was constitutively expressed. We find that tobacco plants transformed with the KN1 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genes & Development Unpaywall

Overexpression of the maize homeo box gene, KNOTTED-1, causes a switch from determinate to indeterminate cell fates.

Sinha, N R; Williams, R E; Hake, S
Genes & Development – May 1, 1993
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Unpaywall
ISSN
0890-9369
DOI
10.1101/gad.7.5.787
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Abstract

Downloaded from genesdev.cshlp.org on October 23, 2021 - Published by Cold Spring Harbor Laboratory Press Overexpression of the maize homeo box gene, KNOTTED-I, causes a switch from determinate to indeterminate cell fates Neelima R. Sinha, Rosalind E. Williams, and Sarah Hake U.S. Department of Agriculture-Agricultural Research Service Plant Gene Expression Center, Albany, California 94710 USA; Department of Plant Biology, University of California, Berkeley, California 94720 USA. The KNOTTED-1 (KN1) locus of maize is defined by dominant mutations that affect leaf cell fates. Transposon tagging led to the isolation of the gene and the discovery that KN1 encodes a homeo domain. Immunolocalization studies showed that in wild-type maize plants, KN1 protein is present in nuclei of apical meristems and immature shoot axes but is down-regulated as lateral organs, such as leaves, are initiated. The protein is not immunohistochemically detectable in wild-type leaves at any stage. In developing leaves of plants carrying the dominant Knl mutation, temporally and spatially restricted ectopic expression of KN1 causes the mutant phenotype. To better understand the function of KN1 in plant development, we sought to determine the phenotype of plants in which KN1 was constitutively expressed. We find that tobacco plants transformed with the KN1

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Genes & Development – Unpaywall

Published: May 1, 1993

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