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References (8)
-
R. Saiki, D. Gelfand, S. Stoffel, S. Scharf, R. Higuchi, G. Horn, K. Mullis, H. Erlich (1988)
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.Science, 239 4839
-
A. Bej, M. Mahbubani, J. Dicesare, R. Atlas (1991)
Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samplesApplied and Environmental Microbiology, 57
-
M. Stern, G. Ames, N. Smith, E. Robinson, C. Higgins (1984)
Repetitive extragenic palindromic sequences: A major component of the bacterial genomeCell, 37
-
S. Mazurier, A. Giessen, K. Heuvelman, K. Wernars (1992)
RAPD analysis of Campylobacter isolates: DNA fingerprinting without the need to purify DNALetters in Applied Microbiology, 14
-
K. Wernars, C. Heuvelman, T. Chakraborty, S. Notermans (1991)
Use of the polymerase chain reaction for direct detection of Listeria monocytogenes in soft cheese.The Journal of applied bacteriology, 70 2
-
(1991)
Polymerase chain reaction-gene probe detection of microorganisms by using filterconcentrated samples -
P. Border, J. Howard, G. Plastow, K. Siggens (1990)
Detection of Listeria species and Listeria monocytogenes using polymerase chain reactionLetters in Applied Microbiology, 11
-
K. Lampel, J. Jagow, M. Trucksess, W. Hill (1990)
Polymerase chain reaction for detection of invasive Shigella flexneri in foodApplied and Environmental Microbiology, 56
- Publisher
- Oxford University Press
- Copyright
- Copyright © 1993 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 0266-8254
- eISSN
- 1472-765X
- DOI
- 10.1111/j.1472-765x.1993.tb00342.x
- Publisher site
- See Article on Publisher Site
Abstract
Oligonucleotide primers designed from repetitive extragenic palindromic (REP) sequences were used to PCR‐amplify Salmonella DNA. Unpurified template DNA, present in crude cell extracts, yielded an essentially identical banding pattern to that arising from the use of purified DNA. However, the PCR product derived from the crude preparations did not survive storage at 4°C. This post‐PCR DNA degradation, attributed to endogenous Salmonella nucleases, was inhibited by the addition of EDTA. or storage at ‐20°C.
Journal
Letters in Applied Microbiology – Oxford University Press
Published: Feb 1, 1993