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Protein deacetylase SIRT1 in the cytoplasm promotes nerve growth factor‐induced neurite outgrowth in PC12 cells

Protein deacetylase SIRT1 in the cytoplasm promotes nerve growth factor‐induced neurite outgrowth... SIRT1, a NAD+‐dependent protein deacetylase, is known to have neural functions. However, despite its cytoplasmic expression in some neural cells, its cytoplasmic function, if any, is unknown. Here we found that PC12 (pheochromocytoma) cells expressed SIRT1 in the cytoplasm. Nerve growth factor (NGF)‐induced neurite outgrowth of these cells was promoted by activators of SIRT1, while inhibitors of SIRT1 or SIRT1‐siRNA significantly inhibited it. The overexpression of a mutant SIRT1 that localised to the cytoplasm but not the nucleus enhanced the NGF‐dependent neurite outgrowth, and a cytoplasmic dominant‐negative SIRT1 suppressed it. Thus, cytoplasmic SIRT1 increases the NGF‐induced neurite outgrowth of PC12 cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Febs Letters Wiley

Protein deacetylase SIRT1 in the cytoplasm promotes nerve growth factor‐induced neurite outgrowth in PC12 cells

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References (18)

Publisher
Wiley
Copyright
© 2015 Federation of European Biochemical Societies
eISSN
1873-3468
DOI
10.1016/j.febslet.2010.04.063
Publisher site
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Abstract

SIRT1, a NAD+‐dependent protein deacetylase, is known to have neural functions. However, despite its cytoplasmic expression in some neural cells, its cytoplasmic function, if any, is unknown. Here we found that PC12 (pheochromocytoma) cells expressed SIRT1 in the cytoplasm. Nerve growth factor (NGF)‐induced neurite outgrowth of these cells was promoted by activators of SIRT1, while inhibitors of SIRT1 or SIRT1‐siRNA significantly inhibited it. The overexpression of a mutant SIRT1 that localised to the cytoplasm but not the nucleus enhanced the NGF‐dependent neurite outgrowth, and a cytoplasmic dominant‐negative SIRT1 suppressed it. Thus, cytoplasmic SIRT1 increases the NGF‐induced neurite outgrowth of PC12 cells.

Journal

Febs Letters – Wiley

Published: Jul 2, 2010

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