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I-(Indole-3-acetyl)-β-D-Glucose, a New Compound in the Metabolism of Indole-3-acetic Acid in Plants

I-(Indole-3-acetyl)-β-D-Glucose, a New Compound in the Metabolism of Indole-3-acetic Acid in Plants STUDYING the fate of exogenously applied indole-3-acetic acid (IAA) in pea epicotyls, we observed, besides indoleacetylaspartic acid1 (IAAsp), a trace amount of a compound which showed definitely indole reactive properties and which was split on alkaline hydrolysis into IAA. The amount of this metabolite proved too small for identification, therefore we carried out a rough survey on one species from 20 plant families using 2-14CIAA in the hope of finding an accumulation of this metabolite. This unknown compound was found in the leaves of the monocotyledonous plant Colchicum neapolitanum Ten. to about 65 per cent of the total amount of IAA taken up, the rest being unchanged IAA (20 per cent) and minor components (Fig. 1, top). IAAsp was detected, if at all, only in very small quantities. In order to isolate a larger amount of this compound, 70 gm. of mature Colchicum leaves were cut into pieces and incubated with 1 litre of a 4 × 10−4 M solution of IAA for 24 hr. with good aeration. The compound was extracted from the leaves with boiling 80 per cent aqueous ethanol; the extract was then concentrated to a small volume and made up to a powder with ‘Hyflo supercel’ and then extracted in a Soxhlet-type extractor for 6 hr. with ether. The ether containing only the IAA was discarded, and extraction continued with ethyl acetate for another 6 hr. This solution was taken to dryness and the residue containing the compound chromatographed as streaks on Whatman No. 3 paper in n-butanol / glacial acetic acid / water (5 : 1 : 2.2, R F 0.56) and in isopropanol / benzine / water (55 : 30 : 11, R F 0.39). By elution and rechromatography between the two solvents, the derivative could be purified and resulted finally in 6.05 mgm. of a highly purified, non-crystalline material. The compound was located on the paper by spraying with p-dimethylaminobenzaldehyde. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Springer Journals

I-(Indole-3-acetyl)-β-D-Glucose, a New Compound in the Metabolism of Indole-3-acetic Acid in Plants

ZENK, M. H.
Nature , Volume 191 (4787) – Jul 29, 1961
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References (6)

Publisher
Springer Journals
Copyright
Copyright © 1961 by Nature Publishing Group
Subject
Science, Humanities and Social Sciences, multidisciplinary; Science, Humanities and Social Sciences, multidisciplinary; Science, multidisciplinary
ISSN
0028-0836
eISSN
1476-4687
DOI
10.1038/191493a0
Publisher site
See Article on Publisher Site

Abstract

STUDYING the fate of exogenously applied indole-3-acetic acid (IAA) in pea epicotyls, we observed, besides indoleacetylaspartic acid1 (IAAsp), a trace amount of a compound which showed definitely indole reactive properties and which was split on alkaline hydrolysis into IAA. The amount of this metabolite proved too small for identification, therefore we carried out a rough survey on one species from 20 plant families using 2-14CIAA in the hope of finding an accumulation of this metabolite. This unknown compound was found in the leaves of the monocotyledonous plant Colchicum neapolitanum Ten. to about 65 per cent of the total amount of IAA taken up, the rest being unchanged IAA (20 per cent) and minor components (Fig. 1, top). IAAsp was detected, if at all, only in very small quantities. In order to isolate a larger amount of this compound, 70 gm. of mature Colchicum leaves were cut into pieces and incubated with 1 litre of a 4 × 10−4 M solution of IAA for 24 hr. with good aeration. The compound was extracted from the leaves with boiling 80 per cent aqueous ethanol; the extract was then concentrated to a small volume and made up to a powder with ‘Hyflo supercel’ and then extracted in a Soxhlet-type extractor for 6 hr. with ether. The ether containing only the IAA was discarded, and extraction continued with ethyl acetate for another 6 hr. This solution was taken to dryness and the residue containing the compound chromatographed as streaks on Whatman No. 3 paper in n-butanol / glacial acetic acid / water (5 : 1 : 2.2, R F 0.56) and in isopropanol / benzine / water (55 : 30 : 11, R F 0.39). By elution and rechromatography between the two solvents, the derivative could be purified and resulted finally in 6.05 mgm. of a highly purified, non-crystalline material. The compound was located on the paper by spraying with p-dimethylaminobenzaldehyde.

Journal

Nature – Springer Journals

Published: Jul 29, 1961

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