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The data base generally known as electrophoretic data is widely acknowledged to be of value to systematics (1, 2, 8, 17, 24, 79, 86). Although starch-gel electrophoresis of enzymes has become the established method of generating this data base, the analysis of electrophoretic data has remained varied and at times openly contested (28, 30, 55, 65, 67, 69, 82, 83). The treatment of these data has produced interesting results that have been perceived to demonstrate severe limitations on the nature and application of this data base at various taxonomic levels. Differing opinions on data treatment often obscure the multistep nature of these treatments. Many studies purporting to compare systematic treatments of electrophoretic data actually confuse the issue by simultaneously varying procedures at several levels , e.g. data transformation, coding, and method of analysis. Several options exist for each step, and these potential differences affect the results of comparative studies in various ways. The historical perspective employed here is essential for understanding the development of issues and the refinement of procedures. This review also assesses the unnecessary limitations that have been imposed on the application of electrophoretic data in systematic studies and identifies the issues and problems that fuel
Annual Review of Ecology, Evolution, and Systematics – Annual Reviews
Published: Nov 1, 1984
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