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The S receptor kinase determines self-incompatibility in Brassica stigma

The S receptor kinase determines self-incompatibility in Brassica stigma The self-incompatibility possessed by Brassica is an intraspecific reproductive barrier by which the stigma rejects self-pollen but accepts non-self-pollen for fertilization. The molecular/biochemical bases of recognition and rejection have been intensively studied. Self-incompatibility in Brassica is sporophytically controlled by the polymorphic S locus 1 . Two tightly linked polymorphic genes at the S locus, S receptor kinase gene (SRK) and S locus glycoprotein gene (SLG), are specifically expressed in the papillar cells of the stigma 2,3,4 , and analyses of self-compatible lines 5,6,7 of Brassica have suggested that together they control stigma function in self-incompatibility interactions. Here we show, by transforming self-incompatible plants of Brassica rapa with an SRK 28 and an SLG 28 transgene separately, that expression of SRK 28 alone, but not SLG 28 alone, conferred the ability to reject self (S 28 )-pollen on the transgenic plants. We also show that the ability of SRK28 to reject S 28 pollen was enhanced by SLG28. We conclude that SRK alone determines S haplotype specificity of the stigma, and that SLG acts to promote a full manifestation of the self-incompatibility response. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Springer Journals

The S receptor kinase determines self-incompatibility in Brassica stigma

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References (34)

Publisher
Springer Journals
Copyright
Copyright © 2000 by Macmillan Magazines Ltd.
Subject
Science, Humanities and Social Sciences, multidisciplinary; Science, Humanities and Social Sciences, multidisciplinary; Science, multidisciplinary
ISSN
0028-0836
eISSN
1476-4687
DOI
10.1038/35002628
Publisher site
See Article on Publisher Site

Abstract

The self-incompatibility possessed by Brassica is an intraspecific reproductive barrier by which the stigma rejects self-pollen but accepts non-self-pollen for fertilization. The molecular/biochemical bases of recognition and rejection have been intensively studied. Self-incompatibility in Brassica is sporophytically controlled by the polymorphic S locus 1 . Two tightly linked polymorphic genes at the S locus, S receptor kinase gene (SRK) and S locus glycoprotein gene (SLG), are specifically expressed in the papillar cells of the stigma 2,3,4 , and analyses of self-compatible lines 5,6,7 of Brassica have suggested that together they control stigma function in self-incompatibility interactions. Here we show, by transforming self-incompatible plants of Brassica rapa with an SRK 28 and an SLG 28 transgene separately, that expression of SRK 28 alone, but not SLG 28 alone, conferred the ability to reject self (S 28 )-pollen on the transgenic plants. We also show that the ability of SRK28 to reject S 28 pollen was enhanced by SLG28. We conclude that SRK alone determines S haplotype specificity of the stigma, and that SLG acts to promote a full manifestation of the self-incompatibility response.

Journal

Nature – Springer Journals

Published: Feb 24, 2000

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