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- Publisher
- Wiley
- Copyright
- Copyright © 2012 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 1552-4922
- eISSN
- 1552-4930
- DOI
- 10.1002/cyto.a.22033
- pmid
- 22411905
- Publisher site
- See Article on Publisher Site
Abstract
Purpose and Appropriate Sample Types This panel was developed to characterize the phenotype of human natural killer (NK) cells from cryopreserved peripheral blood mononuclear cells (PBMC) isolated from ACD or EDTA anticoagulated whole blood or apheresis units (Table 1 ). The application of this panel was to identify changes in NK cell subsets with regard to receptor expression, maturation, homing potential, and activation in the setting of primary HIV‐1 natural infection. However, this panel may be applied to a wide variety of disease and normal conditions to characterize NK cells in humans. The performance of this panel was tested on fresh and frozen PBMC as well as using a whole blood lyse no wash procedure. 1 Summary table for OMIP‐007 Purpose Characterize the phenotype, maturation, and activation of NK cells Species Human Cell types Fresh and cryopreserved PBMC Cross references None Background NK cells are innate effector cells representing ∼10% of circulating lymphocytes with more than 2 billion in circulation at any given time ( 1 ). NK cells are classically defined by the expression of two cellular markers; CD56, the neural cell adhesion molecule (NCAM) and CD16, the Fcγ‐receptor IIIa. These markers (CD56/CD16) allow the discrimination of
Journal
Cytometry Part A – Wiley
Published: Jun 1, 2012
Keywords: ; ; ;