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Chemiluminescence Immunoassay: An Alternative to RIA

Chemiluminescence Immunoassay: An Alternative to RIA Abstract Radioimmunoassay (RIA), since its introduction almost 30 years ago, has been an extremely valuable diagnostic tool due to its excellent sensitivity and specificity. Its main drawback has been the limitations of its radioactive tracer component℄short reagent life, licensing, and disposal problems. Most of the proposed alternatives to RIA have failed to replace it completely, however, due to lack of sensitivity, stability, or molecular size restrictions. Chemiluminescence immumunoassay (CLIA), using acridinium esters as direct replacements for l25I or other isotopes in classic competitive and immunometric assays, shows promise in all respects as a practical alternative. Long shelf-life, simple technology, sensitivity at least as good as RIA, and wide analyte applicability are some of the major advantages of CLIA. To our knowledge, the MAGIC ® Lite System of Ciba-Corning Diagnostics is the first example of such a CLIA system. This content is only available as a PDF. © American Society of Clinical Pathologists http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Laboratory Medicine Oxford University Press

Chemiluminescence Immunoassay: An Alternative to RIA

Dudley, Richard, F.
Laboratory Medicine , Volume 21 (4) – Apr 1, 1990
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Publisher
Oxford University Press
Copyright
© American Society of Clinical Pathologists
ISSN
0007-5027
eISSN
1943-7730
DOI
10.1093/labmed/21.4.216
Publisher site
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Abstract

Abstract Radioimmunoassay (RIA), since its introduction almost 30 years ago, has been an extremely valuable diagnostic tool due to its excellent sensitivity and specificity. Its main drawback has been the limitations of its radioactive tracer component℄short reagent life, licensing, and disposal problems. Most of the proposed alternatives to RIA have failed to replace it completely, however, due to lack of sensitivity, stability, or molecular size restrictions. Chemiluminescence immumunoassay (CLIA), using acridinium esters as direct replacements for l25I or other isotopes in classic competitive and immunometric assays, shows promise in all respects as a practical alternative. Long shelf-life, simple technology, sensitivity at least as good as RIA, and wide analyte applicability are some of the major advantages of CLIA. To our knowledge, the MAGIC ® Lite System of Ciba-Corning Diagnostics is the first example of such a CLIA system. This content is only available as a PDF. © American Society of Clinical Pathologists

Journal

Laboratory Medicine – Oxford University Press

Published: Apr 1, 1990

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