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References (46)
-
Stura Stura, Satterthwait Satterthwait, Calvo Calvo, Kaslow Kaslow, Wilson Wilson (1994)
Reverse screeningActa Crystallogr D, 50
-
H. Kozono, Janicé White, J. Clements, P. Marrack, J. Kappler (1994)
Production of soluble MHC class II proteins with covalently bound single peptidesNature, 369
-
E. Bikoff, Li-Yun Huang, Vasso Episkopou, Joost Meerwijk, Gonald Germain, Elizabeth Robertson (1993)
Defective major histocompatibility complex class II assembly, transport, peptide acquisition, and CD4+ T cell selection in mice lacking invariant chain expressionThe Journal of Experimental Medicine, 177
-
D. Fremont, W. Hendrickson, P. Marrack, J. Kappler (1996)
Structures of an MHC Class II Molecule with Covalently Bound Single PeptidesScience, 272
-
W. Lafuse (1991)
Molecular biology of murine MHC class II genes.Critical reviews in immunology, 11 3-4
-
Rammensee (1995)
MHC ligands and peptide motifs: First listingImmunogenetics, 41
-
(1995)
MHC ligands and peptide -
P. Bjorkman, P. Bjorkman, M. Saper, B. Samraoui, W. Bennett, W. Bennett, J. Strominger, D. Wiley (1987)
Structure of the human class I histocompatibility antigen, HLA-A2Nature, 329
-
R. Germain, L. Hendrix (1991)
MHC class II structure, occupancy and surface expression determined by post-endoplasmic reticulum antigen bindingNature, 353
-
L. Mengle-Gaw, H. Mcdevitt (1985)
Genetics and expression of mouse Ia antigens.Annual review of immunology, 3
-
S. Sadegh-Nasseri, R. Germain (1991)
A role for peptide in determining MHC class II structureNature, 353
-
(1994)
XPREP-Data preparation and reciprocal space evaluu -
R. Martin, H. McFarland, D. McFarlin (1992)
Immunological aspects of demyelinating diseases.Annual review of immunology, 10
-
M. Ridley (1995)
Here, there, everywhereNature, 375
-
F. Carbone, M. Bevan (1989)
Induction of ovalbumin-specific cytotoxic T cells by in vivo peptide immunizationThe Journal of Experimental Medicine, 169
-
A. Howard, G. Gilliland, B. Finzel, T. Poulos, D. Ohlendorf, F. Salemme (1987)
The use of an imaging proportional counter in macromolecular crystallographyJournal of Applied Crystallography, 20
-
C. Scott, K Garcia, F. Carbone, I. Wilson, L. Teyton (1996)
Role of chain pairing for the production of functional soluble IA major histocompatibility complex class II moleculesThe Journal of Experimental Medicine, 183
-
G. Chiocchia, B. Manoury, M. Boissier, C. Fournier (1993)
T cell-targeted immunotherapy in murine collagen-induced arthritis.Clinical and experimental rheumatology, 11 Suppl 9
-
I. Wilson (1996)
Another Twist to MHC-Peptide RecognitionScience, 272
-
B. Nag, S. Arimilli, B. Koukis, E. Rhodes, V. Baichwal, S. Sharma (1994)
Intramolecular charge heterogeneity in purified major histocompatibility class II alpha and beta polypeptide chains.The Journal of biological chemistry, 269 13
-
Masazumi Matsumura, D. Fremont, Per Peterson, Ian Wilson (1992)
Emerging principles for the recognition of peptide antigens by MHC class I molecules.Science, 257 5072
-
M. Saper, P. Bjorkman, D. Wiley (1991)
Refined structure of the human histocompatibility antigen HLA-A2 at 2.6 A resolution.Journal of molecular biology, 219 2
-
P. Ghosh, M. Amaya, E. Mellins, D. Wiley (1995)
The structure of an intermediate in class II MHC maturation: CLIP bound to HLA-DR3Nature, 378
-
J. Jancarik, Sung-Hou Kim (1991)
Sparse matrix sampling: a screening method for crystallization of proteinsJournal of Applied Crystallography, 24
-
(1990)
On the dissociation and reassociation of MHC class 11-foreign peptide complexes -
J. Kappler, B. Skidmore, J. White, P. Marrack (1981)
Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognitionThe Journal of Experimental Medicine, 153
-
M. Matsumura, Y. Saito, M. Jackson, E. Song, Per Peterson (1992)
In vitro peptide binding to soluble empty class I major histocompatibility complex molecules isolated from transfected Drosophila melanogaster cells.The Journal of biological chemistry, 267 33
-
D. Fremont, Enrico Stura, Masazaumi Matsumura, Per Peterson, Ian Wilson (1995)
Crystal structure of an H-2Kb-ovalbumin peptide complex reveals the interplay of primary and secondary anchor positions in the major histocompatibility complex binding groove.Proceedings of the National Academy of Sciences of the United States of America, 92
-
T. Jardetzky, Jerry Brown, J. Gorga, L. Stern, R. Urban, Y. Chi, C. Stauffacher, J. Strominger, D. Wiley (1994)
Three-dimensional structure of a human class II histocompatibility molecule complexed with superantigenNature, 368
-
L. Wicker, J. Todd, L. Peterson (1995)
Genetic control of autoimmune diabetes in the NOD mouse.Annual review of immunology, 13
-
Jerry Brown, T. Jardetzky, J. Gorga, L. Stern, R. Urban, J. Strominger, D. Wiley (1993)
Three-dimensional structure of the human class II histocompatibility antigen HLA-DR1Nature, 364
-
T. Jardetzky, Jerry Brown, J. Gorga, L. Stern, R. Urban, J. Strominger, D. Wiley (1996)
Crystallographic analysis of endogenous peptides associated with HLA-DR1 suggests a common, polyproline II-like conformation for bound peptides.Proceedings of the National Academy of Sciences of the United States of America, 93 2
-
D. Fremont, Masazumi Matsumura, Enrico Stura, Per Peterson, Ian Wilson (1994)
Crystal structures of two viral peptides in complex with murine MHC class I H-2Kb.Science, 257 5072
-
R. Germain (1994)
MHC-dependent antigen processing and peptide presentation: Providing ligands for T lymphocyte activationCell, 76
-
P. Cresswell (1994)
Assembly, transport, and function of MHC class II molecules.Annual review of immunology, 12
-
(1992)
1992b. In vitro -
I. Wilson (1994)
Covalently linked ligand stabilizes expression of heterodimeric receptor.Structure, 2 6
-
Michael Jackson, Elizabeth Song, Young Yang, Per Peterson (1992)
Empty and peptide-containing conformers of class I major histocompatibility complex molecules expressed in Drosophila melanogaster cells.Proceedings of the National Academy of Sciences of the United States of America, 89 24
-
J. Lee, T. Watts (1990)
On the dissociation and reassociation of MHC class II-foreign peptide complexes. Evidence that brief transit through an acidic compartment is not sufficient for binding site regeneration.Journal of immunology, 144 5
-
L. Stern, Jerry Brown, T. Jardetzky, J. Gorga, R. Urban, J. Strominger, D. Wiley (1994)
Crystal structure of the human class II MHC protein HLA-DR1 complexed with an influenza virus peptideNature, 368
-
E. Stura, G. Nemerow, I. Wilson (1992)
Strategies in the crystallization of glycoproteins and protein complexesJournal of Crystal Growth, 122
-
(1981)
Antigen-inducible, H-2-restricted, and H-2 recognition -
L. Stern, D. Wiley (1994)
Antigenic peptide binding by class I and class II histocompatibility proteins.Structure, 2 4
-
(1992)
Seeding techniques -
(1992)
Crystal Science 257:919-927 -
A. Korman, J. Boss, T. Spies, R. Sorrentino, K. Okada, J. Strominger (1985)
Genetic Complexity and Expression of Human Class II Histocompatibility AntigensImmunological Reviews, 85
- Publisher
- Wiley
- Copyright
- Copyright © 1998 The Protein Society
- ISSN
- 0961-8368
- eISSN
- 1469-896X
- DOI
- 10.1002/pro.5560070222
- pmid
- 9521118
- Publisher site
- See Article on Publisher Site
Abstract
Class II Major Histocompatibility (MHC) molecules are cell surface heterodimeric glycoproteins that play a central role in the immune response by presenting peptide antigens for surveillance by T cells. Due to the inherent instability of the class II MHC heterodimer, and its dependence on bound peptide for proper assembly, the production of electrophoretically pure samples of class II MHC proteins in complex with specific peptides has been problematic. A soluble form of the murine class II MHC molecule, I‐Ad, with a leucine zipper tail added to each chain to enhance dimer assembly and secretion, has been produced in Drosophila melanogaster SC2 cells. To facilitate peptide loading, a high affinity ovalbumin peptide was covalently engineered to be attached by a six‐residue linker to the amino terminus of the I‐Adβ chain. This modified I‐Ad molecule was purified using preparative IEF and one fraction, after removal of the leucine zipper tails, produced crystals suitable for X‐ray crystallographic analysis. The protein engineering and purification methods described here should be of general value for the expression of I‐A and other class II MHC‐peptide complexes.
Journal
Protein Science – Wiley
Published: Feb 1, 1998