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Measurement of mouse brain glucose utilization in vivo using [U-14C]glucose

Measurement of mouse brain glucose utilization in vivo using [U-14C]glucose The rate of [2-14C]glucose uptake has been used as an indication of the status of energy consumption by the rat brain, but the cost of this radiolabel can be prohibitive and the surgical manipulation involved in published methods is extensive. A method for measuring glucose utilization in vivo in mouse brain with [U-14C]glucose is described in this article. Glucose consumption in whole mouse brain obtained with [U-14C]glucose or [2-14C]glucose was 0.650±0.022 and 0.716±0.36 nmol/mg/min, respectively. In all instances the rate obtained with the uniformly labeled isotope was somewhat lower than that found with [2-14C]glucose. The rate of glucose utilization measured with either isotope was significantly depressed in sodium pentobarbital anesthetized mice. The method described here is advantageous because [U-14C]glucose is substantially less expensive than [2-14C]glucose and surgical intervention is avoided. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neurochemical Research Springer Journals

Measurement of mouse brain glucose utilization in vivo using [U-14C]glucose

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References (16)

Publisher
Springer Journals
Copyright
Copyright
Subject
Biomedicine; Neurosciences; Neurochemistry; Biochemistry, general; Cell Biology; Neurology
ISSN
0364-3190
eISSN
1573-6903
DOI
10.1007/BF00964460
Publisher site
See Article on Publisher Site

Abstract

The rate of [2-14C]glucose uptake has been used as an indication of the status of energy consumption by the rat brain, but the cost of this radiolabel can be prohibitive and the surgical manipulation involved in published methods is extensive. A method for measuring glucose utilization in vivo in mouse brain with [U-14C]glucose is described in this article. Glucose consumption in whole mouse brain obtained with [U-14C]glucose or [2-14C]glucose was 0.650±0.022 and 0.716±0.36 nmol/mg/min, respectively. In all instances the rate obtained with the uniformly labeled isotope was somewhat lower than that found with [2-14C]glucose. The rate of glucose utilization measured with either isotope was significantly depressed in sodium pentobarbital anesthetized mice. The method described here is advantageous because [U-14C]glucose is substantially less expensive than [2-14C]glucose and surgical intervention is avoided.

Journal

Neurochemical Research – Springer Journals

Published: Dec 24, 2004

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