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High resolution studies on the pattern of induced exchanges in the human karyotype

High resolution studies on the pattern of induced exchanges in the human karyotype The pattern of breakage and exchange induced by X-irradiation of human lymphocytes at G1 has been analysed in PHA transformed cultures at X1 metaphase in cells treated with trypsin. All the events observed occurred in interband segments. Moreover, as far as the autosomes were concerned, these events appear to be at random in relation to trypsin interband length but give some indication of non-randomness when total chromosome length is considered. The X and Y chromosomes, on the other hand, show far fewer breaks than would be expected whichever criterion is adopted, and in particular appear to be isolated from the autosomes with respect to the occurrence of exchange events. — The analysis of specific break points in relation to trypsin banding sequences, makes it clear that conclusions regarding chromosome rearrangements based solely on conventional preparations may be misleading. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Chromosoma Springer Journals

High resolution studies on the pattern of induced exchanges in the human karyotype

Seabright, Marina
Chromosoma , Volume 40 (4) – Sep 16, 2004
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References (23)

Publisher
Springer Journals
Copyright
Copyright © 1973 by Springer-Verlag
Subject
Life Sciences; Cell Biology; Developmental Biology; Biochemistry, general; Human Genetics; Animal Genetics and Genomics; Eukaryotic Microbiology
ISSN
0009-5915
eISSN
1432-0886
DOI
10.1007/BF00399426
Publisher site
See Article on Publisher Site

Abstract

The pattern of breakage and exchange induced by X-irradiation of human lymphocytes at G1 has been analysed in PHA transformed cultures at X1 metaphase in cells treated with trypsin. All the events observed occurred in interband segments. Moreover, as far as the autosomes were concerned, these events appear to be at random in relation to trypsin interband length but give some indication of non-randomness when total chromosome length is considered. The X and Y chromosomes, on the other hand, show far fewer breaks than would be expected whichever criterion is adopted, and in particular appear to be isolated from the autosomes with respect to the occurrence of exchange events. — The analysis of specific break points in relation to trypsin banding sequences, makes it clear that conclusions regarding chromosome rearrangements based solely on conventional preparations may be misleading.

Journal

Chromosoma – Springer Journals

Published: Sep 16, 2004

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