Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Conditional deletion of Xist disrupts histone macroH2A localization but not maintenance of X inactivation

Conditional deletion of Xist disrupts histone macroH2A localization but not maintenance of X... © 1999 Nature America Inc. • http://genetics.nature.com correspondence FISH with probes for the X-linked genes Conditional deletion of Xist disrupts Pgk1 and Hprt as well as Xist. In wild-type 2lox fibroblasts and fibroblasts carrying Xist , histone macroH2A localization but we detected a Pgk1 signal coming from the active X (Xa) in approximately 50% of not maintenance of X inactivation nuclei and an Hprt signal in 60%. After Cre-mediated deletion of Xist, we did not n mammalian females, dosage compen- 1a, and data not shown). We generated see biallelic expression of X-linked genes or 2lox I sation is achieved by X-chromosome mice carrying the Xist allele and mouse a signal in a higher percentage of nuclei, inactivation. Xist has an essential role in the embryonic fibroblasts with the genotypes suggesting that the mutant Xi remains 1,2 2lox/+ 2lox/Y 2lox/∆ initiation of the inactivation process . Xist Xist , Xist and Xist by breed- transcriptionally silent (Fig. 1c,d). 2lox is transcribed exclusively from the inactive ing mice carrying Xist with mice carry- Xi resembles constitutive heterochro- 2 ∆ 12 X (Xi; refs 3,4), and the RNA stays in the ing a null Xist allele (Xist ). Because Xist is matin http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Genetics Springer Journals

Conditional deletion of Xist disrupts histone macroH2A localization but not maintenance of X inactivation

Download PDF
2 pages

Loading next page...
 
/lp/springer-journals/conditional-deletion-of-xist-disrupts-histone-macroh2a-localization-MyAhhyObgl

References (15)

Publisher
Springer Journals
Copyright
Copyright © 1999 by Nature America Inc.
Subject
Biomedicine; Biomedicine, general; Human Genetics; Cancer Research; Agriculture; Gene Function; Animal Genetics and Genomics
ISSN
1061-4036
eISSN
1546-1718
DOI
10.1038/11887
Publisher site
See Article on Publisher Site

Abstract

© 1999 Nature America Inc. • http://genetics.nature.com correspondence FISH with probes for the X-linked genes Conditional deletion of Xist disrupts Pgk1 and Hprt as well as Xist. In wild-type 2lox fibroblasts and fibroblasts carrying Xist , histone macroH2A localization but we detected a Pgk1 signal coming from the active X (Xa) in approximately 50% of not maintenance of X inactivation nuclei and an Hprt signal in 60%. After Cre-mediated deletion of Xist, we did not n mammalian females, dosage compen- 1a, and data not shown). We generated see biallelic expression of X-linked genes or 2lox I sation is achieved by X-chromosome mice carrying the Xist allele and mouse a signal in a higher percentage of nuclei, inactivation. Xist has an essential role in the embryonic fibroblasts with the genotypes suggesting that the mutant Xi remains 1,2 2lox/+ 2lox/Y 2lox/∆ initiation of the inactivation process . Xist Xist , Xist and Xist by breed- transcriptionally silent (Fig. 1c,d). 2lox is transcribed exclusively from the inactive ing mice carrying Xist with mice carry- Xi resembles constitutive heterochro- 2 ∆ 12 X (Xi; refs 3,4), and the RNA stays in the ing a null Xist allele (Xist ). Because Xist is matin

Journal

Nature Genetics – Springer Journals

Published: Aug 1, 1999

There are no references for this article.