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THE RELEASE OF HYALURONIDASE FROM GUINEA-PIG SPERMATOZOA DURING THE COURSE OF THE NORMAL ACROSOME REACTION IN VITRO

THE RELEASE OF HYALURONIDASE FROM GUINEA-PIG SPERMATOZOA DURING THE COURSE OF THE NORMAL ACROSOME... Hamster spermatozoa incubated in heat-pretreated blood serum release 55 to 60% of their total hyaluronidase activity by the end of 1 hr of incubation; little increase in activity is found in the incubation medium at later time intervals up to 4 hr (Rogers & Morton, 1973; Talbot & Franklin, 1974b). This release occurs well before and independently of a normal acrosome reaction, i.e. detachment of the acrosome from motile spermatozoa as observed with phase-contrast microscopy (Talbot & Franklin, 1974b). Vesiculation between the outer acrosomal membrane and plasma membrane does not occur before the surge of hyaluronidase release which takes place between time zero and 1 hr of incubation (Talbot & Franklin, 1974b).Lewis & Ketchel (1972a, 1973) demonstrated that the release of hyaluronidase from rabbit spermatozoa incubated in postovulatory uterine fluid is "related to the completion http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Reproduction Bioscientifica

THE RELEASE OF HYALURONIDASE FROM GUINEA-PIG SPERMATOZOA DURING THE COURSE OF THE NORMAL ACROSOME REACTION IN VITRO

Reproduction , Volume 39 (2) – Aug 1, 1974

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Publisher
Bioscientifica
Copyright
Copyright © 1974 The Authors. All Rights Reserved.
ISSN
1470-1626
eISSN
1741-7899
DOI
10.1530/jrf.0.0390429
Publisher site
See Article on Publisher Site

Abstract

Hamster spermatozoa incubated in heat-pretreated blood serum release 55 to 60% of their total hyaluronidase activity by the end of 1 hr of incubation; little increase in activity is found in the incubation medium at later time intervals up to 4 hr (Rogers & Morton, 1973; Talbot & Franklin, 1974b). This release occurs well before and independently of a normal acrosome reaction, i.e. detachment of the acrosome from motile spermatozoa as observed with phase-contrast microscopy (Talbot & Franklin, 1974b). Vesiculation between the outer acrosomal membrane and plasma membrane does not occur before the surge of hyaluronidase release which takes place between time zero and 1 hr of incubation (Talbot & Franklin, 1974b).Lewis & Ketchel (1972a, 1973) demonstrated that the release of hyaluronidase from rabbit spermatozoa incubated in postovulatory uterine fluid is "related to the completion

Journal

ReproductionBioscientifica

Published: Aug 1, 1974

There are no references for this article.