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Cell Cycling of Astrocytes and Their Precursors in Primary Cultures: A Mevalonate Requirement Identified in Late G 1 , but Before the G 1 /S Transition, Involves Polypeptides

Cell Cycling of Astrocytes and Their Precursors in Primary Cultures: A Mevalonate Requirement... Abstract: The relationship between mevalonate and cell cycling was investigated in developing glial cells. Primary cultures of newborn rat brains were serum‐depleted (0.1%, vol/vol) for 48 h on days 4–6 in vitro, then returned to 10% calf serum (time 0). After 48 h, 70–80% of the cells were glial fibrillary acidic protein (GFAP)‐negative by indirect immunofluorescence; 79 ± 7% were GFAP‐positive after an additional 3 days. Serum shift‐up resulted in 12 h of quiescence, and then by 20 h (S phase) in increased proportions of cells synthesizing DNA (from 15 ± 6% to 75 ± 4% by bromodeoxyuridine immunofluorescence at 12 h and 20 h, respectively) and rates of DNA synthesis (42 ± 6 versus 380 ± 32 cpm/μg of protein/h of (3H)thymidine uptake). Addition of mevalonate (25 mM) for 30 min at 10 h reversed the inhibition of DNA synthesis apparent with mevinolin (150 μM), an inhibitor of mevalonate synthesis, present from time 0. Cycloheximide added simultaneously with mevalonate prevented this reversal of inhibition. To cause arrest at G1/S, cultures were exposed to hydroxyurea between 10 and 22 h. By 3 h after hydroxyurea removal, bromodeoxyuridine‐labeled nuclei increased from 0% to 75 ± 9%, and DNA synthesis increased 10‐fold. Mevinolin failed to inhibit these increases. Thus, primary astroglial precursors stimulated to progress through the cell cycle express a mevalonate requirement in late G1, but before the G1/S transition. The effect of mevalonate was characterized further as being brief (30 min) and as requiring polypeptides. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Cell Cycling of Astrocytes and Their Precursors in Primary Cultures: A Mevalonate Requirement Identified in Late G 1 , but Before the G 1 /S Transition, Involves Polypeptides

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References (111)

Publisher
Wiley
Copyright
Copyright © 1991 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1991.tb02029.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: The relationship between mevalonate and cell cycling was investigated in developing glial cells. Primary cultures of newborn rat brains were serum‐depleted (0.1%, vol/vol) for 48 h on days 4–6 in vitro, then returned to 10% calf serum (time 0). After 48 h, 70–80% of the cells were glial fibrillary acidic protein (GFAP)‐negative by indirect immunofluorescence; 79 ± 7% were GFAP‐positive after an additional 3 days. Serum shift‐up resulted in 12 h of quiescence, and then by 20 h (S phase) in increased proportions of cells synthesizing DNA (from 15 ± 6% to 75 ± 4% by bromodeoxyuridine immunofluorescence at 12 h and 20 h, respectively) and rates of DNA synthesis (42 ± 6 versus 380 ± 32 cpm/μg of protein/h of (3H)thymidine uptake). Addition of mevalonate (25 mM) for 30 min at 10 h reversed the inhibition of DNA synthesis apparent with mevinolin (150 μM), an inhibitor of mevalonate synthesis, present from time 0. Cycloheximide added simultaneously with mevalonate prevented this reversal of inhibition. To cause arrest at G1/S, cultures were exposed to hydroxyurea between 10 and 22 h. By 3 h after hydroxyurea removal, bromodeoxyuridine‐labeled nuclei increased from 0% to 75 ± 9%, and DNA synthesis increased 10‐fold. Mevinolin failed to inhibit these increases. Thus, primary astroglial precursors stimulated to progress through the cell cycle express a mevalonate requirement in late G1, but before the G1/S transition. The effect of mevalonate was characterized further as being brief (30 min) and as requiring polypeptides.

Journal

Journal of Neurochemistry – Wiley

Published: Mar 1, 1991

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