Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

Adsorption of 14-Labeled Gramicidin S on Cell of Bacteria

Adsorption of 14-Labeled Gramicidin S on Cell of Bacteria Abstract Gramicidin S (GS) containing 14C-labelled proline was synthesized by a solid-phase method, and the labeled GS dihydrochloride was obtained as crystals. The labeled GS exhibited same antibacterial activity as natural GS. Strains sensitive to GS (B. subtilis and S. aureus) and an insensitive strain (E. coli) were treated with the labeled GS, and the amount of the labeled GS adsorbed on the cells was measured. GS was adsorbed rapidly on the cells of the sensitive strains; the amount adsorbed increased linearly with GS concentration up to 1–1.5μg/ml, and at a lower rate at above 1.5μ/ml. GS molecules covered most of the cell surface at the minimum inhibitory concentration of 1.5μ/ml, the number of molecules adsorbed per cell was 1.3–1.4×105. No GS was adsorbed by the insensitive strain. This content is only available as a PDF. © 1981, by the Japanese Biochemical Society http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Biochemistry Oxford University Press

Adsorption of 14-Labeled Gramicidin S on Cell of Bacteria

Download PDF
5 pages

Loading next page...
 
/lp/oxford-university-press/adsorption-of-14-labeled-gramicidin-s-on-cell-of-bacteria-L0lfdxY0U2

References (0)

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Oxford University Press
Copyright
© 1981, by the Japanese Biochemical Society
ISSN
0021-924X
eISSN
1756-2651
DOI
10.1093/oxfordjournals.jbchem.a133560
Publisher site
See Article on Publisher Site

Abstract

Abstract Gramicidin S (GS) containing 14C-labelled proline was synthesized by a solid-phase method, and the labeled GS dihydrochloride was obtained as crystals. The labeled GS exhibited same antibacterial activity as natural GS. Strains sensitive to GS (B. subtilis and S. aureus) and an insensitive strain (E. coli) were treated with the labeled GS, and the amount of the labeled GS adsorbed on the cells was measured. GS was adsorbed rapidly on the cells of the sensitive strains; the amount adsorbed increased linearly with GS concentration up to 1–1.5μg/ml, and at a lower rate at above 1.5μ/ml. GS molecules covered most of the cell surface at the minimum inhibitory concentration of 1.5μ/ml, the number of molecules adsorbed per cell was 1.3–1.4×105. No GS was adsorbed by the insensitive strain. This content is only available as a PDF. © 1981, by the Japanese Biochemical Society

Journal

The Journal of Biochemistry – Oxford University Press

Published: Oct 1, 1981

There are no references for this article.