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References (21)
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FIXATION OF THE CENTRAL NERVOUS SYSTEM FROM ELECTRON MICROSCOPY BY ALDEHYDE PERFUSION. I. PRESERVATION WITH ALDEHYDE PERFUSATES VERSUS DIRECT PERFUSION WITH OSMIUM TETROXIDE WITH SPECIAL REFERENCE TO MEMBRANES AND THE EXTRACELLULAR SPACE.Journal of ultrastructure research, 12
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S. Palay, S. Mcgee-Russell, S. Gordon, M. Grillo (1962)
FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDEThe Journal of Cell Biology, 12
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Embedding Media — Old and New -
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A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPYThe Journal of Cell Biology, 25
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Observations on the blood‐testis barrier of the rat and on the physiological compartmentalization of the seminiferous epithelium, 3
- Publisher
- Wiley
- Copyright
- "Copyright © 1977 Wiley Subscription Services, Inc., A Wiley Company"
- ISSN
- 1932-8486
- eISSN
- 1932-8494
- DOI
- 10.1002/ar.1091880304
- pmid
- 332010
- Publisher site
- See Article on Publisher Site
Abstract
A reliable and uniform vascular perfusion fixation method for the testis has been developed by using an initial washout solution containing a vasodilator and an anticoagulant. This is followed by a brief fixation with a sodium phosphate buffered formaldehyde‐glutaraldehyde solution of conventional strength, and then a second more concentrated aldehyde fixative solution containing picric acid. The method takes into account some of the unique features of the vascular supply of the male genital tract for its favorable perfusion and fixation. The advantages of this method are: (1) consistently favorable preservation of the testis; (2) simple and inexpensive apparatus; and (3) stable and relatively innocuous stock solutions.
Journal
The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology – Wiley
Published: Jul 1, 1977