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- Publisher
- Wiley
- Copyright
- Copyright © 2000 John Wiley & Sons, Ltd.
- ISSN
- 1076-5174
- eISSN
- 1096-9888
- DOI
- 10.1002/(SICI)1096-9888(200005)35:5<595::AID-JMS965>3.0.CO;2-D
- pmid
- 10800048
- Publisher site
- See Article on Publisher Site
Abstract
Structural characterization of the glycerophosphoethanolamine (GPE) molecule as a lithiated adduct ion by collisionally activated dissociation (CAD) tandem mass spectrometry with electrospray ionization is described. Abundant fragment ions reflecting polar head group and fatty acid constituents were observed in the product ion spectrum of GPE, which permits an unambiguous structural determination, including the regiospecificity of fatty acyl substituents. The pathways leading to the formation of fragment ions are proposed. The suggested mechanisms are supported by the tandem mass spectra of various deuterated analogs and source CAD of GPE followed by CAD tandem mass spectrometry. Identification of GPE molecular species and specific GPE subclasses in a biological mixture by tandem mass spectrometry with various constant neutral loss scannings is also described. Copyright © 2000 John Wiley & Sons, Ltd.
Journal
Journal of Mass Spectrometry (Incorp Biological Mass Spectrometry) – Wiley
Published: May 1, 2000