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Estimation and manipulation of glutathione levels in prepuberal mouse ovaries and ova:Relevance to sperm nucleus transformation in the fertilized egg

Estimation and manipulation of glutathione levels in prepuberal mouse ovaries and ova:Relevance... Glutathione (GSH), the major low‐molecular‐weight thiol in mammalian cells, is believed to be a necessary factor for the transformation of the disulfide‐stabilized sperm nucleus into the male pronucleus after fertilization. Its concentration in mouse ova, isolated from the ampulla of the oviduct after hormone‐induced superovulation of 3–4‐week‐old mice, has been determined by an enzymic cycling microassay. The level found was 1.80 pmol per ovum. Mean ovum diameter was estimated as 71–72 μm, indicating a GSH concentration of 9–10 mM in the mouse egg. Administration of L‐buthionine S, R‐sulfoximine, an inhibitor of GSH biosynthesis, during the 2 days preceding ovulation, reduced ovum GSH content below 0.20 pmol (<1.0 mM). The mean GSH concentration of the hormone‐stimulated ovaries was reduced from 3.2 mM to 0.2 mM under these conditions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Reproduction & Development Wiley

Estimation and manipulation of glutathione levels in prepuberal mouse ovaries and ova:Relevance to sperm nucleus transformation in the fertilized egg

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References (25)

Publisher
Wiley
Copyright
Copyright © 1986 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1040-452X
eISSN
1098-2795
DOI
10.1002/mrd.1120140310
Publisher site
See Article on Publisher Site

Abstract

Glutathione (GSH), the major low‐molecular‐weight thiol in mammalian cells, is believed to be a necessary factor for the transformation of the disulfide‐stabilized sperm nucleus into the male pronucleus after fertilization. Its concentration in mouse ova, isolated from the ampulla of the oviduct after hormone‐induced superovulation of 3–4‐week‐old mice, has been determined by an enzymic cycling microassay. The level found was 1.80 pmol per ovum. Mean ovum diameter was estimated as 71–72 μm, indicating a GSH concentration of 9–10 mM in the mouse egg. Administration of L‐buthionine S, R‐sulfoximine, an inhibitor of GSH biosynthesis, during the 2 days preceding ovulation, reduced ovum GSH content below 0.20 pmol (<1.0 mM). The mean GSH concentration of the hormone‐stimulated ovaries was reduced from 3.2 mM to 0.2 mM under these conditions.

Journal

Molecular Reproduction & Development – Wiley

Published: Jul 1, 1986

Keywords: ; ; ; ;

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