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Unbound Plasma Cortisol in Pregnant and Enovid-E Treated Women as Determined by Ultrafiltration

Unbound Plasma Cortisol in Pregnant and Enovid-E Treated Women as Determined by Ultrafiltration Abstract Unbound plasma cortisol concentrations were determined in nonpregnant, pregnant and Enovid-E treated women. A method is described for the measurement of the unbound fraction using ultrafiltration by centrifugation at 37 C to obtain a filtrate of 1/10 the volume of the plasma sample. Tracer amounts of cortisol-3H were added to determine the per cent of cortisol filterable. Total cortisol concentrations were measured by a fluorometric technique. The mean unbound or ultrafilterable cortisol concentration for the pregnant women was 2.47 μg/100 ml, which is significantly higher (p<.001) than the mean of 1.00 μg/100 ml for the nonpregnant, and 1.31 μg/100 ml for the Enovid-E treated women. High concentrations of total plasma cortisol were present, as expected, in both the pregnant and the Enovid-E treated women, but the latter group had a significantly lower (p<.001) per cent filterable cortisol of 4.3% as compared to 6.3% for the pregnant women, and 7.6% for the nonpregnant women. The implications of the findings in pregnant women are briefly discussed. This content is only available as a PDF. Author notes Supported by USPHS Grant AM-05748 from the National Institute of Arthritis and Metabolic Diseases. 1 Enovid-E contains 0.1 mg ethynylestradiol 3-methyl ether and 2.5 mg norethynodrel. Enovid, 10 mg, contains 0.15 mg ethynylestradiol 3-methyl ether and 9.85 mg norethynodrel, a synthetic progestational agent. Copyright © 1969 by The Endocrine Society http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Clinical Endocrinology and Metabolism Oxford University Press

Unbound Plasma Cortisol in Pregnant and Enovid-E Treated Women as Determined by Ultrafiltration

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Publisher
Oxford University Press
Copyright
Copyright © 1969 by The Endocrine Society
ISSN
0021-972X
eISSN
1945-7197
DOI
10.1210/jcem-29-4-563
Publisher site
See Article on Publisher Site

Abstract

Abstract Unbound plasma cortisol concentrations were determined in nonpregnant, pregnant and Enovid-E treated women. A method is described for the measurement of the unbound fraction using ultrafiltration by centrifugation at 37 C to obtain a filtrate of 1/10 the volume of the plasma sample. Tracer amounts of cortisol-3H were added to determine the per cent of cortisol filterable. Total cortisol concentrations were measured by a fluorometric technique. The mean unbound or ultrafilterable cortisol concentration for the pregnant women was 2.47 μg/100 ml, which is significantly higher (p<.001) than the mean of 1.00 μg/100 ml for the nonpregnant, and 1.31 μg/100 ml for the Enovid-E treated women. High concentrations of total plasma cortisol were present, as expected, in both the pregnant and the Enovid-E treated women, but the latter group had a significantly lower (p<.001) per cent filterable cortisol of 4.3% as compared to 6.3% for the pregnant women, and 7.6% for the nonpregnant women. The implications of the findings in pregnant women are briefly discussed. This content is only available as a PDF. Author notes Supported by USPHS Grant AM-05748 from the National Institute of Arthritis and Metabolic Diseases. 1 Enovid-E contains 0.1 mg ethynylestradiol 3-methyl ether and 2.5 mg norethynodrel. Enovid, 10 mg, contains 0.15 mg ethynylestradiol 3-methyl ether and 9.85 mg norethynodrel, a synthetic progestational agent. Copyright © 1969 by The Endocrine Society

Journal

Journal of Clinical Endocrinology and Metabolism – Oxford University Press

Published: Apr 1, 1969

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