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Gene structure and expression profile of Manduca sexta prophenoloxidase‐activating proteinase‐3 (PAP‐3), an immune protein containing two clip domains

Gene structure and expression profile of Manduca sexta prophenoloxidase‐activating proteinase‐3... Prophenoloxidase‐activating proteinase‐3 (PAP‐3) is a component of the defence system in Manduca sexta. We have isolated genomic clones and elucidated the organization of this gene. The 3′ end of exon 2, the entire exon 3 and the 5′ end of exon 4 encode the two amino‐terminal clip domains. Southern blot analysis suggested a single copy of the PAP‐3 gene in the genome. We identified several putative immune‐responsive elements in the upstream region. The PAP‐3 gene is not highly expressed in the fat body during larval development until the wandering stage begins. The mRNA level is high in the epithelium, fat body and haemocytes. Tissue‐specific alternative splicing occurs in the fat body and trachea. A bacterial injection markedly induced the gene expression in the fat body and haemocytes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Insect Molecular Biology Wiley

Gene structure and expression profile of Manduca sexta prophenoloxidase‐activating proteinase‐3 (PAP‐3), an immune protein containing two clip domains

Insect Molecular Biology , Volume 14 (4) – Aug 1, 2005

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References (35)

Publisher
Wiley
Copyright
Copyright © 2005 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0962-1075
eISSN
1365-2583
DOI
10.1111/j.1365-2583.2005.00574.x
pmid
16033436
Publisher site
See Article on Publisher Site

Abstract

Prophenoloxidase‐activating proteinase‐3 (PAP‐3) is a component of the defence system in Manduca sexta. We have isolated genomic clones and elucidated the organization of this gene. The 3′ end of exon 2, the entire exon 3 and the 5′ end of exon 4 encode the two amino‐terminal clip domains. Southern blot analysis suggested a single copy of the PAP‐3 gene in the genome. We identified several putative immune‐responsive elements in the upstream region. The PAP‐3 gene is not highly expressed in the fat body during larval development until the wandering stage begins. The mRNA level is high in the epithelium, fat body and haemocytes. Tissue‐specific alternative splicing occurs in the fat body and trachea. A bacterial injection markedly induced the gene expression in the fat body and haemocytes.

Journal

Insect Molecular BiologyWiley

Published: Aug 1, 2005

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