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Reproducibility of Differential Centrifugation Experiments in Tissue Fractionation

Reproducibility of Differential Centrifugation Experiments in Tissue Fractionation DURING the past few years, differential centrifugation of tissue homogenates has become one of the major techniques of cytology and biochemistry alike. Most of those who have applied this new technique describe the conditions of centrifugation by mentioning only the duration of operation and the centrifugal field prevailing in the middle (or at the bottom) of the tube; the type of centrifuge used is sometimes also mentioned. However, the information thus supplied is not sufficient to enable other workers to reproduce the conditions, especially when another type of instrument is to be used. This fact may be partly responsible for the wide variations which are sometimes encountered between results obtained in different laboratories. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Springer Journals

Reproducibility of Differential Centrifugation Experiments in Tissue Fractionation

DE DUVE, C.; BERTHET, J.
Nature , Volume 172 (4390) – Dec 19, 1953
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Reproducibility of Differential Centrifugation Experiments in Tissue Fractionation

Abstract

DURING the past few years, differential centrifugation of tissue homogenates has become one of the major techniques of cytology and biochemistry alike. Most of those who have applied this new technique describe the conditions of centrifugation by mentioning only the duration of operation and the centrifugal field prevailing in the middle (or at the bottom) of the tube; the type of centrifuge used is sometimes also mentioned. However, the information thus supplied is not sufficient to enable...
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References (2)

Publisher
Springer Journals
Copyright
Copyright © Springer Nature Limited 1953
Subject
Science, Humanities and Social Sciences, multidisciplinary; Science, Humanities and Social Sciences, multidisciplinary; Science, multidisciplinary
ISSN
0028-0836
eISSN
1476-4687
DOI
10.1038/1721142a0
Publisher site
See Article on Publisher Site

Abstract

DURING the past few years, differential centrifugation of tissue homogenates has become one of the major techniques of cytology and biochemistry alike. Most of those who have applied this new technique describe the conditions of centrifugation by mentioning only the duration of operation and the centrifugal field prevailing in the middle (or at the bottom) of the tube; the type of centrifuge used is sometimes also mentioned. However, the information thus supplied is not sufficient to enable other workers to reproduce the conditions, especially when another type of instrument is to be used. This fact may be partly responsible for the wide variations which are sometimes encountered between results obtained in different laboratories.

Journal

Nature – Springer Journals

Published: Dec 19, 1953

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