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Acetylation of H2AZ Lys 14 is associated with genome-wide gene activity in yeast

Acetylation of H2AZ Lys 14 is associated with genome-wide gene activity in yeast Downloaded from genesdev.cshlp.org on October 6, 2021 - Published by Cold Spring Harbor Laboratory Press Acetylation of H2AZ Lys 14 is associated with genome-wide gene activity in yeast 1 1 2 1,3 Catherine B. Millar, Feng Xu, Kangling Zhang, and Michael Grunstein Department of Biological Chemistry, Geffen School of Medicine and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA; Mass Spectrometry Facility, Department of Chemistry, University of California, Riverside, California 92521, USA Histone variants and their post-translational modifications help regulate chromosomal functions. Htz1 is an evolutionarily conserved H2A variant found at several promoters in the yeast Saccharomyces cerevisiae.In this study, we undertook a genome-wide analysis of Htz1 and its modifications in yeast. Using mass spectrometric analysis, we determined that Htz1 is acetylated at Lys 3, Lys 8, Lys 10, and Lys 14 within its N-terminal tail, with K14 being the most abundant acetylated site. ChIP and microarray analysis were then used to compare the location of Htz1-K14 acetylation to that of Htz1 genome-wide. The data presented here demonstrate that while Htz1 is associated preferentially with the promoters of repressed genes, K14 acetylation is enriched at the promoters of active genes, and requires two known histone http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genes & Development Unpaywall

Acetylation of H2AZ Lys 14 is associated with genome-wide gene activity in yeast

Millar, Catherine B.; Xu, Feng; Zhang, Kangling; Grunstein, Michael
Genes & Development – Mar 15, 2006
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Unpaywall
ISSN
0890-9369
DOI
10.1101/gad.1395506
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Abstract

Downloaded from genesdev.cshlp.org on October 6, 2021 - Published by Cold Spring Harbor Laboratory Press Acetylation of H2AZ Lys 14 is associated with genome-wide gene activity in yeast 1 1 2 1,3 Catherine B. Millar, Feng Xu, Kangling Zhang, and Michael Grunstein Department of Biological Chemistry, Geffen School of Medicine and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA; Mass Spectrometry Facility, Department of Chemistry, University of California, Riverside, California 92521, USA Histone variants and their post-translational modifications help regulate chromosomal functions. Htz1 is an evolutionarily conserved H2A variant found at several promoters in the yeast Saccharomyces cerevisiae.In this study, we undertook a genome-wide analysis of Htz1 and its modifications in yeast. Using mass spectrometric analysis, we determined that Htz1 is acetylated at Lys 3, Lys 8, Lys 10, and Lys 14 within its N-terminal tail, with K14 being the most abundant acetylated site. ChIP and microarray analysis were then used to compare the location of Htz1-K14 acetylation to that of Htz1 genome-wide. The data presented here demonstrate that while Htz1 is associated preferentially with the promoters of repressed genes, K14 acetylation is enriched at the promoters of active genes, and requires two known histone

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Genes & Development – Unpaywall

Published: Mar 15, 2006

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