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Phosphatidylinositol-3-OH kinases are Rab5 effectors

Phosphatidylinositol-3-OH kinases are Rab5 effectors brief communications Phosphatidylinositol-3-OH kinases are Rab5 effectors Savvas Christoforidis*†, Marta Miaczynska*†, Keith Ashman†, Matthias Wilm†, Liyun Zhao*, Shu-Chin Yip‡, Michael D. Waterfield§, Jonathan M. Backer‡ and Marino Zerial*†¶ *Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse, 01307 Dresden, Germany †European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany ‡Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA §Ludwig Institute of Cancer Research, University College London, Riding House Street, London W1P 8BT, UK ¶e-mail: [email protected] hosphatidylinositol-3-OH kinases (PI(3)Ks) constitute a fam- coupling the local production of phosphoinositides to the selective ily of lipid-modifying enzymes that are involved in signal recruitment of Rab5 effector proteins. transduction, cytoskeletal organization and membrane We used matrix-assisted laser-desorption-ionization mass spec- transport . Three different classes of PI(3)K have been described, trometry (MALDI-MS) to identify the proteins purified on the basis which differ in their specificities for phosphatidylinositol, phos- of a specific interaction with the active form of Rab5 (ref. 5). Sur- phatidylinositol-4-phosphate and phosphatidylinositol-4,5- prisingly, among these proteins we identified the regulatory subunit bisphosphate substrates, as well as in their regulation. PI(3)Ks are of class-I PI(3)Ks, p85a, and confirmed its identity by western blot- needed for receptor http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Cell Biology Springer Journals

Phosphatidylinositol-3-OH kinases are Rab5 effectors

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References (18)

Publisher
Springer Journals
Copyright
Copyright © 1999 by Macmillan Magazines Ltd.
Subject
Life Sciences; Life Sciences, general; Cell Biology; Cancer Research; Developmental Biology; Stem Cells
ISSN
1465-7392
eISSN
1476-4679
DOI
10.1038/12075
Publisher site
See Article on Publisher Site

Abstract

brief communications Phosphatidylinositol-3-OH kinases are Rab5 effectors Savvas Christoforidis*†, Marta Miaczynska*†, Keith Ashman†, Matthias Wilm†, Liyun Zhao*, Shu-Chin Yip‡, Michael D. Waterfield§, Jonathan M. Backer‡ and Marino Zerial*†¶ *Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse, 01307 Dresden, Germany †European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany ‡Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA §Ludwig Institute of Cancer Research, University College London, Riding House Street, London W1P 8BT, UK ¶e-mail: [email protected] hosphatidylinositol-3-OH kinases (PI(3)Ks) constitute a fam- coupling the local production of phosphoinositides to the selective ily of lipid-modifying enzymes that are involved in signal recruitment of Rab5 effector proteins. transduction, cytoskeletal organization and membrane We used matrix-assisted laser-desorption-ionization mass spec- transport . Three different classes of PI(3)K have been described, trometry (MALDI-MS) to identify the proteins purified on the basis which differ in their specificities for phosphatidylinositol, phos- of a specific interaction with the active form of Rab5 (ref. 5). Sur- phatidylinositol-4-phosphate and phosphatidylinositol-4,5- prisingly, among these proteins we identified the regulatory subunit bisphosphate substrates, as well as in their regulation. PI(3)Ks are of class-I PI(3)Ks, p85a, and confirmed its identity by western blot- needed for receptor

Journal

Nature Cell Biology – Springer Journals

Published: Jul 15, 1999

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