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Non-thermal plasma suppresses bacterial colonization on skin wound and promotes wound healing in mice

Non-thermal plasma suppresses bacterial colonization on skin wound and promotes wound healing in... The present study evaluated the effect of non-thermal plasma on skin wound healing in BalB/c mice. Two 6-mm wounds along the both sides of the spine were created on the back of each mouse (n=80) by using a punch biopsy. The mice were assigned randomly into two groups, with 40 animals in each group: a non-thermal plasma group in which the mice were treated with the non-thermal plasma; a control group in which the mice were left to heal naturally. Wound healing was evaluated on postoperative days (POD) 4, 7, 10 and 14 (n=5 per group in each POD) by percentage of wound closure. The mice was euthanized on POD 1, 4, 7, 10, 14, 21, 28 and 35 (n=1 in each POD). The wounds were removed, routinely fixed, paraffin-embedded, sectioned and HE-stained. A modified scoring system was used to evaluate the wounds. The results showed that acute inflammation peaked on POD 4 in non-thermal plasma group, earlier than in control group in which acute inflammation reached a peak on POD 7, and the acute inflammation scores were much lower in non-thermal group than in control group on POD 7 (P<0.05). The amount of granular tissue was greater on POD 4 and 7 in non-thermal group than in control group (P<0.05). The re-epithelialization score and the neovasularization score were increased significantly in non-thermal group when compared with control group on POD 7 and 10 (P<0.05 for all). The count of bacterial colonies was 103 CFU/mL on POD 4 and <20 CFU/mL on POD 7, significantly lower than that in control group (109 CFU/mL on POD 4 and >1012 CFU/mL on the POD 7) (P<0.05). It was suggested that the non-thermal plasma facilitates the wound healing by suppressing bacterial colonization. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Huazhong University of Science and Technology [Medical Sciences] Springer Journals

Non-thermal plasma suppresses bacterial colonization on skin wound and promotes wound healing in mice

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References (25)

Publisher
Springer Journals
Copyright
Copyright © 2011 by Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg
Subject
Medicine & Public Health; Medicine/Public Health, general
ISSN
1672-0733
eISSN
1993-1352
DOI
10.1007/s11596-011-0387-2
pmid
21671184
Publisher site
See Article on Publisher Site

Abstract

The present study evaluated the effect of non-thermal plasma on skin wound healing in BalB/c mice. Two 6-mm wounds along the both sides of the spine were created on the back of each mouse (n=80) by using a punch biopsy. The mice were assigned randomly into two groups, with 40 animals in each group: a non-thermal plasma group in which the mice were treated with the non-thermal plasma; a control group in which the mice were left to heal naturally. Wound healing was evaluated on postoperative days (POD) 4, 7, 10 and 14 (n=5 per group in each POD) by percentage of wound closure. The mice was euthanized on POD 1, 4, 7, 10, 14, 21, 28 and 35 (n=1 in each POD). The wounds were removed, routinely fixed, paraffin-embedded, sectioned and HE-stained. A modified scoring system was used to evaluate the wounds. The results showed that acute inflammation peaked on POD 4 in non-thermal plasma group, earlier than in control group in which acute inflammation reached a peak on POD 7, and the acute inflammation scores were much lower in non-thermal group than in control group on POD 7 (P<0.05). The amount of granular tissue was greater on POD 4 and 7 in non-thermal group than in control group (P<0.05). The re-epithelialization score and the neovasularization score were increased significantly in non-thermal group when compared with control group on POD 7 and 10 (P<0.05 for all). The count of bacterial colonies was 103 CFU/mL on POD 4 and <20 CFU/mL on POD 7, significantly lower than that in control group (109 CFU/mL on POD 4 and >1012 CFU/mL on the POD 7) (P<0.05). It was suggested that the non-thermal plasma facilitates the wound healing by suppressing bacterial colonization.

Journal

Journal of Huazhong University of Science and Technology [Medical Sciences] – Springer Journals

Published: Jun 14, 2011

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