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Isolation, immortalization, and characterization of a human breast epithelial cell line with stem cell properties

Isolation, immortalization, and characterization of a human breast epithelial cell line with stem... Downloaded from genesdev.cshlp.org on October 23, 2021 - Published by Cold Spring Harbor Laboratory Press Isolation, immortalization, and characterization of a human breast epithelial cell line with stem cell properties 1 1 1,2 2 Thorarinn Gudjonsson, Rene ´ Villadsen, Helga Lind Nielsen, Lone Rønnov-Jessen, 3 1,4 Mina J. Bissell, and Ole William Petersen Structural Cell Biology Unit, Institute of Medical Anatomy, The Panum Institute, DK-2200 Copenhagen N, Denmark; 2 3 Zoophysiological Laboratory, The August Krogh Institute, DK-2100 Copenhagen Ø, Denmark; Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA The epithelial compartment of the human breast comprises two distinct lineages: the luminal epithelial and the myoepithelial lineage. We have shown previously that a subset of the luminal epithelial cells could convert to myoepithelial cells in culture signifying the possible existence of a progenitor cell. We therefore set out to identify and isolate the putative precursor in the luminal epithelial compartment. Using cell surface markers and immunomagnetic sorting, we isolated two luminal epithelial cell populations from primary cultures of reduction mammoplasties. The major population coexpresses sialomucin (MUC ) and epithelial-specific antigen (ESA ) whereas the minor population has a suprabasal position and expresses − + http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genes & Development Unpaywall

Isolation, immortalization, and characterization of a human breast epithelial cell line with stem cell properties

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ISSN
0890-9369
DOI
10.1101/gad.952602
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Abstract

Downloaded from genesdev.cshlp.org on October 23, 2021 - Published by Cold Spring Harbor Laboratory Press Isolation, immortalization, and characterization of a human breast epithelial cell line with stem cell properties 1 1 1,2 2 Thorarinn Gudjonsson, Rene ´ Villadsen, Helga Lind Nielsen, Lone Rønnov-Jessen, 3 1,4 Mina J. Bissell, and Ole William Petersen Structural Cell Biology Unit, Institute of Medical Anatomy, The Panum Institute, DK-2200 Copenhagen N, Denmark; 2 3 Zoophysiological Laboratory, The August Krogh Institute, DK-2100 Copenhagen Ø, Denmark; Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA The epithelial compartment of the human breast comprises two distinct lineages: the luminal epithelial and the myoepithelial lineage. We have shown previously that a subset of the luminal epithelial cells could convert to myoepithelial cells in culture signifying the possible existence of a progenitor cell. We therefore set out to identify and isolate the putative precursor in the luminal epithelial compartment. Using cell surface markers and immunomagnetic sorting, we isolated two luminal epithelial cell populations from primary cultures of reduction mammoplasties. The major population coexpresses sialomucin (MUC ) and epithelial-specific antigen (ESA ) whereas the minor population has a suprabasal position and expresses − +

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Genes & Development – Unpaywall

Published: Mar 15, 2002

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