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- Publisher
- Wiley
- Copyright
- © John Wiley and Sons
- ISSN
- 1941-7322
- eISSN
- 1938-8969
- DOI
- 10.1002/9780470151808.sc02a08s6
- Publisher site
- See Article on Publisher Site
Abstract
This unit describes the isolation of hematopoietic stem cells (HSCs) from the mouse placenta. The placenta was recently identified as an important hematopoietic site that generates HSCs de novo and provides a transitory niche for a large pool of HSCs during midgestation. This protocol includes a dissection technique for murine placenta, the mechanical and enzymatic steps of placental tissue dissociation, and phenotypical identification and isolation of HSCs. It also contains a method for immunohistochemical analysis of placenta tissue sections to visualize developing HSCs in the placenta. Curr. Protoc. Stem Cell Biol. 6:2A.1.1‐2A.1.14. © 2008 by John Wiley & Sons, Inc.
Journal
Current Protocols in Stem Cell Biology – Wiley
Published: Sep 1, 2008
Keywords: hematopoietic stem cell (HSC); placenta; fetal; dissection; flow cytometry; FACS sorting; fixed‐frozen sections; immunohistochemistry