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micro‐checker: software for identifying and correcting genotyping errors in microsatellite data

micro‐checker: software for identifying and correcting genotyping errors in microsatellite data DNA degradation, low DNA concentrations and primer‐site mutations may result in the incorrect assignment of microsatellite genotypes, potentially biasing population genetic analyses. micro‐checker is windows®‐based software that tests the genotyping of microsatellites from diploid populations. The program aids identification of genotyping errors due to nonamplified alleles (null alleles), short allele dominance (large allele dropout) and the scoring of stutter peaks, and also detects typographic errors. micro‐checker estimates the frequency of null alleles and, importantly, can adjust the allele and genotype frequencies of the amplified alleles, permitting their use in further population genetic analysis. micro‐checker can be freely downloaded from http://www.microchecker.hull.ac.uk/. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Ecology Resources Wiley

micro‐checker: software for identifying and correcting genotyping errors in microsatellite data

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References (16)

Publisher
Wiley
Copyright
Copyright © 2004 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1755-098X
eISSN
1755-0998
DOI
10.1111/j.1471-8286.2004.00684.x
Publisher site
See Article on Publisher Site

Abstract

DNA degradation, low DNA concentrations and primer‐site mutations may result in the incorrect assignment of microsatellite genotypes, potentially biasing population genetic analyses. micro‐checker is windows®‐based software that tests the genotyping of microsatellites from diploid populations. The program aids identification of genotyping errors due to nonamplified alleles (null alleles), short allele dominance (large allele dropout) and the scoring of stutter peaks, and also detects typographic errors. micro‐checker estimates the frequency of null alleles and, importantly, can adjust the allele and genotype frequencies of the amplified alleles, permitting their use in further population genetic analysis. micro‐checker can be freely downloaded from http://www.microchecker.hull.ac.uk/.

Journal

Molecular Ecology ResourcesWiley

Published: Sep 1, 2004

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