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Nucleotide Sequence of cDNA Clone Encoding a Metallothionein-Like Protein from Chinese Cabbage

Nucleotide Sequence of cDNA Clone Encoding a Metallothionein-Like Protein from Chinese Cabbage Downloaded from https://academic.oup.com/plphys/article/108/2/863/6069662 by DeepDyve user on 13 July 2021 Plant Physiol. (1995) 108: 863 Plant Gene Register Nucleotide Sequence of cDNA Clone Encoding a Metallothionein-Like Protein from Chinese Cabbage Hyun Uk Kim*, Jae Beom Kim, Choong Hyo Yun, Seok Kwon Kang, and Tae Young Chung Genetics Division, Agricultural Biotechnology Institute, Rural Development Administration, Suwon 441 -707, Korea (H.U.K., J.B.K., C.H.Y., T.Y.C.); and Department of Agricultura1 Biology, Seoul National University, Suwon 441-774, Korea (S.K.K.) Metallothionein is a low mo1 wt, Cys-rich metal-binding Table 1. Characteristics of pBIF98 protein that is resistant to high levels of toxic metal ions by Organ ism: means of detoxification (Steffens, 1990). A full-length Brassica campestris L. ssp. pekinensis inflorescence. cDNA (pBIF 98) encoding the metallothionein-like protein Location of Cene: was isolated from the inflorescence cDNA library of Chi- Nuclear encoded. nese cabbage (Brassica campestris L. ssp. pekinesis). The Cene Function: Metallothionein-like protein. nucleotide sequence has been determined using the Techniques: dideoxynucleotide chain-termination method (Sanger et lsolation of cDNA clone from hZapll, subcloning into Bluescript al., 1977) and the amino acid sequence deduced (Table I). I1 SK- and dideoxy sequencing of both strands. The cDNA sequence is composed of 505 nucleotides, a Clone Type: putative open reading frame of 240 bp, a 5’ nontranslated cDNA, full length, pBIF98. sequence of 3 bp, and a 3’ noncoding region of 247 bp. The Features of cDNA Structure: 3’ noncoding region contains the sequence AATATA 20 bp 240-bp open reading frame, 3-bp 5’ untranslated region. 247-bp upstream from the polyadenylation site, which is homolo- 3’ untranslated region, polyadenylated tail present, possible polyadenylation signal, AATATA at position 471 . gous to the polyadenylation signal (AATAAA). Thr puta- Features of Protein: tive open reading frame encodes a protein of 80 residues Open reading frame of 80 amino acids, mo1 wt = 8,108.82. It (mo1 wt = 8108.82), which contains 14 Cys residues and contains 14 Cys residues, six Cys-X-Cys or Cys-Cys clusters three Cys-X-Cys or Cys-Cys clusters at the terminal region present. of the polypeptide. The regions of the Cys-X-Cys cluster at G/C Content: each terminal region are metal-binding domains. The do- 55.4% in coding region. mains are separated by an intervening sequence of amino acids like other metallothionein proteins (de Miranda et al., 1990). Comparison of this predicted protein with the pro- tein identification resource protein data base (DNASIS LITERATURE ClTED software) shows 91.4, 47.4, 44.7, and 44.6% homology at the amino acid leve1 with metallothionein proteins of de Framond AJ (1991) A metallothionein-like gene from maize Arabidopsis thaliana (Protein Identification Resource acces- (Zeu mnys) cloning and characterization. FEBS Lett 290 103-106 de Miranda JR, Thomas MA, Thurman DA, Tomsett AB (1990) sion No. S18069), garden pea (Evans et al., 1990), maize Metallothionein genes from the flowering plant Mimulus gutta- (de Framond, 1991), and barley (Okumura et al., 1991), tus. FEBS Lett 260: 277-280 respectively . Evans IM, Gatehouse LN, Gatehouse JA, Robinson NJ, Croy RRD (1990) A gene from pea (Pisum sutivum L.) with homology to metallothionein genes. FEBS Lett 262: 29-32 Received November 17, 1994; accepted December 21, 1994. Okumura N, Nishizawa NK, Umehara Y, Mori S (1991) An iron Copyright Clearance Center: 0032-0889/95/108/0863/01. deficiency-specific cDNA from barley roots having two homol- The GenBank accession number for the sequence reported in this ogous cysteine-rich MT domains. Plant Mo1 Biol 17: 531-533 article is L31940. Sanger F, Nicklen S, Coulson R (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74 5463-5468 * Corresponding author; e-mail [email protected]; fax 82-331- Steffens JC (1990) The heavy metal-binding peptides of plants. 294-1072. Annu Rev Plant Mo1 Biol 41: 553-575 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Physiology Oxford University Press

Nucleotide Sequence of cDNA Clone Encoding a Metallothionein-Like Protein from Chinese Cabbage

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Publisher
Oxford University Press
Copyright
Copyright © 2021 American Society of Plant Biologists
ISSN
0032-0889
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1532-2548
DOI
10.1104/pp.108.2.863
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Abstract

Downloaded from https://academic.oup.com/plphys/article/108/2/863/6069662 by DeepDyve user on 13 July 2021 Plant Physiol. (1995) 108: 863 Plant Gene Register Nucleotide Sequence of cDNA Clone Encoding a Metallothionein-Like Protein from Chinese Cabbage Hyun Uk Kim*, Jae Beom Kim, Choong Hyo Yun, Seok Kwon Kang, and Tae Young Chung Genetics Division, Agricultural Biotechnology Institute, Rural Development Administration, Suwon 441 -707, Korea (H.U.K., J.B.K., C.H.Y., T.Y.C.); and Department of Agricultura1 Biology, Seoul National University, Suwon 441-774, Korea (S.K.K.) Metallothionein is a low mo1 wt, Cys-rich metal-binding Table 1. Characteristics of pBIF98 protein that is resistant to high levels of toxic metal ions by Organ ism: means of detoxification (Steffens, 1990). A full-length Brassica campestris L. ssp. pekinensis inflorescence. cDNA (pBIF 98) encoding the metallothionein-like protein Location of Cene: was isolated from the inflorescence cDNA library of Chi- Nuclear encoded. nese cabbage (Brassica campestris L. ssp. pekinesis). The Cene Function: Metallothionein-like protein. nucleotide sequence has been determined using the Techniques: dideoxynucleotide chain-termination method (Sanger et lsolation of cDNA clone from hZapll, subcloning into Bluescript al., 1977) and the amino acid sequence deduced (Table I). I1 SK- and dideoxy sequencing of both strands. The cDNA sequence is composed of 505 nucleotides, a Clone Type: putative open reading frame of 240 bp, a 5’ nontranslated cDNA, full length, pBIF98. sequence of 3 bp, and a 3’ noncoding region of 247 bp. The Features of cDNA Structure: 3’ noncoding region contains the sequence AATATA 20 bp 240-bp open reading frame, 3-bp 5’ untranslated region. 247-bp upstream from the polyadenylation site, which is homolo- 3’ untranslated region, polyadenylated tail present, possible polyadenylation signal, AATATA at position 471 . gous to the polyadenylation signal (AATAAA). Thr puta- Features of Protein: tive open reading frame encodes a protein of 80 residues Open reading frame of 80 amino acids, mo1 wt = 8,108.82. It (mo1 wt = 8108.82), which contains 14 Cys residues and contains 14 Cys residues, six Cys-X-Cys or Cys-Cys clusters three Cys-X-Cys or Cys-Cys clusters at the terminal region present. of the polypeptide. The regions of the Cys-X-Cys cluster at G/C Content: each terminal region are metal-binding domains. The do- 55.4% in coding region. mains are separated by an intervening sequence of amino acids like other metallothionein proteins (de Miranda et al., 1990). Comparison of this predicted protein with the pro- tein identification resource protein data base (DNASIS LITERATURE ClTED software) shows 91.4, 47.4, 44.7, and 44.6% homology at the amino acid leve1 with metallothionein proteins of de Framond AJ (1991) A metallothionein-like gene from maize Arabidopsis thaliana (Protein Identification Resource acces- (Zeu mnys) cloning and characterization. FEBS Lett 290 103-106 de Miranda JR, Thomas MA, Thurman DA, Tomsett AB (1990) sion No. S18069), garden pea (Evans et al., 1990), maize Metallothionein genes from the flowering plant Mimulus gutta- (de Framond, 1991), and barley (Okumura et al., 1991), tus. FEBS Lett 260: 277-280 respectively . Evans IM, Gatehouse LN, Gatehouse JA, Robinson NJ, Croy RRD (1990) A gene from pea (Pisum sutivum L.) with homology to metallothionein genes. FEBS Lett 262: 29-32 Received November 17, 1994; accepted December 21, 1994. Okumura N, Nishizawa NK, Umehara Y, Mori S (1991) An iron Copyright Clearance Center: 0032-0889/95/108/0863/01. deficiency-specific cDNA from barley roots having two homol- The GenBank accession number for the sequence reported in this ogous cysteine-rich MT domains. Plant Mo1 Biol 17: 531-533 article is L31940. Sanger F, Nicklen S, Coulson R (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74 5463-5468 * Corresponding author; e-mail [email protected]; fax 82-331- Steffens JC (1990) The heavy metal-binding peptides of plants. 294-1072. Annu Rev Plant Mo1 Biol 41: 553-575

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Plant Physiology – Oxford University Press

Published: Jun 1, 1995

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