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References (38)
-
S. Lurie (1979)
Differential Ion Stimulation of Plasmalemma Adenosine Triphosphatase from Leaf Epidermis and Mesophyll of Nicotiana rustica L.Plant physiology, 63 5
-
W. J. Woude, C. A. Lembi, D. J. Morré, J. I. Kindinger, L Ordin (1974)
β‐Glucansynthelases of plasma membrane and Golgi apparatus form onion stem, 54
-
P. Kjellbom, C. Larsson (1984)
Preparation and polypeptide composition of chlorophyll‐free plasma membranes from leaves of light‐grown spinach and barleyPhysiologia Plantarum, 62
-
J. M. Lord (1983)
Isolation of Membranes and Organdies from Plant Cells -
S. Dowdy, W. Stanley (1983)
Wiley Series in Probability and Mathematical Statistics -
S. Mandala, L. Taiz (1985)
Partial purification of a tonoplast ATPase from corn coleoptiles.Plant physiology, 78 2
-
K. Raschke, R. Hedrich (1985)
Simultaneous and independent effects of abscisic acid on stomata and the photosynthetic apparatus in whole leaves, 163
-
I. Møller, W. Lin (1986)
MEMBRANE-BOUND NAD(P)H DEHYDROGENASES IN HIGHER PLANT CELLSAnnual Review of Plant Biology, 37
-
S. Yoshida, T. Kawata, M. Uemura, T. Niki (1986)
Properties of plasma membrane isolated from chilling‐sensitive seedlings of Vigna radiata L, 80
-
T. Hodges, R. Leonard (1974)
Purification of a plasma membrane-bound adenosine triphosphatase from plant roots.Methods in enzymology, 32
-
K. Raschke (1979)
Encyclopedia of Plant Physiology. New Series, 7
-
S. Widell, T. Lundborg, C. Larsson (1982)
Plasma membranes from oats prepared by partition in an aqueous polymer two‐phase system, 70
-
K. Kasamo (1979)
Characterization of membrane-bound Mg++-activated ATPase isolated from the lower epidermis of tobacco leavesPlant and Cell Physiology, 20
-
K. Kasamo (1979)
Effect of abscisic acid on membrane-bound epidermal ATPase from tobacco leavesPlant and Cell Physiology, 20
-
A. Nejidat, N. Roth-Bejerano, C. Itai (1986)
K, Mg-ATPase activity in guard cells of Commelina communisPhysiologia Plantarum, 68
-
Christer Larsson, P. Kjellbom, S. Widell, Tomas Lundborg (1984)
Sidedness of plant plasma membrane vesicles purified by partitioning in aqueous two‐phase systemsFEBS Letters, 171
-
A. Raghavendra, I. Rao, V. Das (1976)
Adenosine triphosphatase in epidermal tissue of Commelina benghalensis: Possible involvement of isozymes in stomatal movementPlant Science Letters, 7
-
Shizuo Yoshida, Takefumi Kawata, Matsuo Uemura, T. Niki (1986)
Properties of Plasma Membrane Isolated from Chilling-Sensitive Etiolated Seedlings of Vigna radiata L.Plant physiology, 80 1
-
W. C. Pierce, D. L. Hendrix (1979)
Utilization of enzyme markers to determine the localization of plasma membrane from Pisum epicotyls on sucrose gradients, 146
-
A. Memon, M. Sommarin, A. Kylin (1987)
Plasmalemma from the roots of cucumber: Isolation by two-phase partitioning and characterizationPhysiologia Plantarum, 69
-
R. Serrano (1983)
Purification and reconstitution of the proton-pumping ATPase of fungal and plant plasma membranes.Archives of biochemistry and biophysics, 227 1
-
J. Widholm (1972)
The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells.Stain technology, 47 4
-
C. Larsson (1985)
Modern Methods of Plant Analysis. New Series, 1
-
W. Woude, Carole Lembi, D. Morré, Jaunita Kindinger, L. Ordin (1974)
beta-Glucan Synthetases of Plasma Membrane and Golgi Apparatus from Onion Stem.Plant physiology, 54 3
-
P. Lanzetta, L. Alvarez, P. Reinach, O. Candia (1979)
An improved assay for nanomole amounts of inorganic phosphate.Analytical biochemistry, 100 1
-
F. Rasi-Caldogno, M. Pugliarello (1985)
Fusicoccin stimulates the H+-ATPase of plasmalemma in isolated membrane vesicles from radish.Biochemical and biophysical research communications, 133 1
-
G. Anthon, R. Spanswick (1986)
Purification and properties of the h-translocating ATPase from the plasma membrane of tomato roots.Plant physiology, 81 4
-
U. Laemmli (1970)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 227
-
H. Sze (1983)
Proton-pumping adenosine triphosphatase in membrane vesicles of tobacco callus: Sensitivity to vanadate and K+Biochimica et Biophysica Acta, 732
-
D. Arnon (1949)
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.Plant physiology, 24 1
-
K. Shimazaki, N. Kondo (1987)
Plasma Membrane H+-ATPase in Guard-Cell Protoplasts from Vicia faba L.Plant and Cell Physiology, 28
-
M. Fricker, C. Willmer (1987)
Vanadate Sensitive ATPase and Phosphatase Activity in Guard Cell Protoplasts of CommelinaJournal of Experimental Botany, 38
-
M. Sommarin, T. Lundborg, A. Kylin (1985)
Comparison of K,MgATPases in purified plasmalemma from wheat and oat. – Substrate specificities and effects of pH, temperature and inhibitorsPhysiologia Plantarum, 65
-
H. Sze (1983)
Proton‐pumping adenosine triphosphatase in membrane vesicles of tobacco callus, 732
-
C. Hornberg, E. W. Weiler (1984)
High affinity binding sites at the plasmalemma of Vicia faba guard cells, 310
-
C. Hornberg, E. Weiler (1984)
High-affinity binding sites for abscisic acid on the plasmalemma of Vicia faba guard cellsNature, 310
-
S. Widell, T. Lundborg, C. Larsson (1982)
Plasma membranes from oats prepared by partition in an aqueous polymer two-phase system : on the use of light-induced cytochrome B reduction as a marker for the plasma membrane.Plant physiology, 70 5
-
M. Feyerabend, E. W. Weiler (1988)
Characterization and localization of fusicoccin binding sites in leaf tissue of Vicia faba probed with a novel radio‐ligand
- Publisher
- Wiley
- Copyright
- Copyright © 1988 Wiley Subscription Services
- ISSN
- 0031-9317
- eISSN
- 1399-3054
- DOI
- 10.1111/j.1399-3054.1988.tb05834.x
- Publisher site
- See Article on Publisher Site
Abstract
Plasmalemma‐rich microsomal vesicles were prepared from whole leaf and acid‐washed epidermal tissue of Vicia faba L. cv. Osnabrücker Markt by aqueous two‐phase partitioning in dextran T‐500 and polyethylenglycol 1350 aqueous phases. These vesicles were tightly sealed and predominantly right‐side out, and contained a K+ ‐stimulated, mg2+‐dependent and vanadate‐sensitive ATPase. The enzyme from both tissues exhibited nearly identical properties: pH optimum 6.4, Km for ATP 0.60 mM(whole leaf) and 0.67 mM (epidermis). Vmax ‐480 nmol (mg protein)1 min1 (whole leaf) and 510 nmol (mg protein)1 min1 (epidermis), I50 (Na3,VO4) 7.5 μM (whole leaf) and 15 μM (epidermis). The enzyme was not inhibited by NO3(50 mM)or sodium azide (I mM). DCCD (20 μM) reduced enzyme activity to 50% (whole leaf) and 58% (epidermis), gramicidin S (20 μM) to 36% (whole leaf) and 41%(epidermis). Ca2+ inhibited the ATPase [I50, C2+: 0.5 mM(whole leaf) and 0.8 mM(epidermis)]. Ca2+ inhibited the ATPase [I50, C2+ 0.5 mM(whole leaf) und 0.8 (epidermis)]. The vanadate‐sensitive ATPase from whole leaf and epidermal tissue was slightly but significantly stimulated by fusicoccin (FC) at a concentration (0.13 μM) promoting stomatal opening. The stimulation was not seen in the solubilized ATPase. Stomata of the cultivar used here were insensitive lo (±)ABA up to 2 μM level which is effective in most other cultivars and species. Likewise, at this concentration no effect of ABA on the activity of the epidermal ATPase was observed. The data are discussed with respect to the interaction of FC and ABA with the ATPase.
Journal
Physiologia Plantarum – Wiley
Published: Jan 1, 1988
Keywords: ; ; ; ; ; ; ; ; ;