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Enrichment of the stages of the seminiferous epithelium in vitamin A-replaced–vitamin A-deficient rats

Enrichment of the stages of the seminiferous epithelium in vitamin A-replaced–vitamin A-deficient... Summary. Morphometric study revealed that, at 40 days after the start of vitamin A replacement, A1 spermatogonia and preleptotene spermatocytes appeared in more than 70% of the whole mounts of seminiferous tubules of vitamin A-deficient rats. By 42 days, the appearance of these cell types was reduced by 50%, and A2 and A3 spermatogonia were predominant. By 46 days, A1–A3 spermatogonia appeared in <30% of the tubular length while A4, intermediate and B spermatogonia became the major cell types in the basement compartment of seminiferous tubules. The predominance of spermatogonia noted at given times was corroborated by higher frequencies of tubular cross-sections of stages in which that particular type of spermatogonium resides. These results indicate that seminiferous tubules of vitamin A-replaced–vitamin A-deficient rats are 'enriched' for particular stages. Tracing the development of [3H]thymidine-labelled preleptotene spermatocytes revealed normal kinetics of germ cell differentiation in these animals. Furthermore, the spermatogonial proliferations in the vitamin A-replaced–vitamin A-deficient rats were quantitatively normal. We suggest that vitamin A replacement may result in temporal suppression of the differentiation of A2–B spermatogonia, leading to a stimulation or synchronization of certain groups of undifferentiating spermatogonia which undergo active proliferation simultaneously. These synchronized populations of spermatogonia continue to proliferate and differentiate, thus resulting in the stage-enrichments noted at later times.Keywords: vitamin A deficiency; spermatogenesis; seminiferous epithelium; stage enrichment; rat http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Reproduction Bioscientifica

Enrichment of the stages of the seminiferous epithelium in vitamin A-replaced–vitamin A-deficient rats

Huang, H. F. S.; Marshall, G. R.; Nieschlag, E.
Reproduction , Volume 88 (1) – Jan 1, 1990
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Publisher
Bioscientifica
Copyright
Copyright © 1990 The Authors. All Rights Reserved.
ISSN
1470-1626
eISSN
1741-7899
DOI
10.1530/jrf.0.0880051
Publisher site
See Article on Publisher Site

Abstract

Summary. Morphometric study revealed that, at 40 days after the start of vitamin A replacement, A1 spermatogonia and preleptotene spermatocytes appeared in more than 70% of the whole mounts of seminiferous tubules of vitamin A-deficient rats. By 42 days, the appearance of these cell types was reduced by 50%, and A2 and A3 spermatogonia were predominant. By 46 days, A1–A3 spermatogonia appeared in <30% of the tubular length while A4, intermediate and B spermatogonia became the major cell types in the basement compartment of seminiferous tubules. The predominance of spermatogonia noted at given times was corroborated by higher frequencies of tubular cross-sections of stages in which that particular type of spermatogonium resides. These results indicate that seminiferous tubules of vitamin A-replaced–vitamin A-deficient rats are 'enriched' for particular stages. Tracing the development of [3H]thymidine-labelled preleptotene spermatocytes revealed normal kinetics of germ cell differentiation in these animals. Furthermore, the spermatogonial proliferations in the vitamin A-replaced–vitamin A-deficient rats were quantitatively normal. We suggest that vitamin A replacement may result in temporal suppression of the differentiation of A2–B spermatogonia, leading to a stimulation or synchronization of certain groups of undifferentiating spermatogonia which undergo active proliferation simultaneously. These synchronized populations of spermatogonia continue to proliferate and differentiate, thus resulting in the stage-enrichments noted at later times.Keywords: vitamin A deficiency; spermatogenesis; seminiferous epithelium; stage enrichment; rat

Journal

Reproduction – Bioscientifica

Published: Jan 1, 1990

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