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Neurosteroids Allopregnanolone Sulfate and Pregnanolone Sulfate Have Diverse Effect on the &agr; Subunit of the Neuronal Voltage-gated Sodium Channels Na v v v v Xenopus

Neurosteroids Allopregnanolone Sulfate and Pregnanolone Sulfate Have Diverse Effect on the &agr;... Neurosteroids Allopregnanolone Sulfate and Pregnanolone Sulfate Have Diverse Effect on the α Subunit of the Neuronal Voltage-gated Sodium Channels Na 1.2, Na 1.6, Na 1.7, and Na 1.8 Expressed v v v v in Xenopus Oocytes Takafumi Horishita, M.D., Ph.D., Nobuyuki Yanagihara, Ph.D., Susumu Ueno, M.D., Ph.D., Yuka Sudo, Ph.D., Yasuhito Uezono, M.D., Ph.D., Dan Okura, M.D., Tomoko Minami, M.D., Takashi Kawasaki, M.D., Ph.D., Takeyoshi Sata, M.D., Ph.D. ABSTRACT Background: The neurosteroids allopregnanolone and pregnanolone are potent positive modulators of γ-aminobutyric acid type A receptors. Antinociceptive effects of allopregnanolone have attracted much attention because recent reports have indi- cated the potential of allopregnanolone as a therapeutic agent for refractory pain. However, the analgesic mechanisms of allo- pregnanolone are still unclear. Voltage-gated sodium channels (Na ) are thought to play important roles in inflammatory and neuropathic pain, but there have been few investigations on the effects of allopregnanolone on sodium channels. Methods: Using voltage-clamp techniques, the effects of allopregnanolone sulfate (APAS) and pregnanolone sulfate (PAS) on sodium current were examined in Xenopus oocytes expressing Na 1.2, Na 1.6, Na 1.7, and Na 1.8 α subunits. v v v v Results: APAS suppressed sodium currents of Na 1.2, Na 1.6, and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Anesthesiology Wolters Kluwer Health

Neurosteroids Allopregnanolone Sulfate and Pregnanolone Sulfate Have Diverse Effect on the &agr; Subunit of the Neuronal Voltage-gated Sodium Channels Na v v v v Xenopus

Sata, Takeyoshi
Anesthesiology , Volume 121 (3) – Sep 1, 2014
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References (47)

Copyright
Copyright © 2014, the American Society of Anesthesiologists, Inc. Lippincott Williams & Wilkins.
ISSN
0003-3022
eISSN
1528-1175
DOI
10.1097/ALN.0000000000000296
pmid
24809977
Publisher site
See Article on Publisher Site

Abstract

Neurosteroids Allopregnanolone Sulfate and Pregnanolone Sulfate Have Diverse Effect on the α Subunit of the Neuronal Voltage-gated Sodium Channels Na 1.2, Na 1.6, Na 1.7, and Na 1.8 Expressed v v v v in Xenopus Oocytes Takafumi Horishita, M.D., Ph.D., Nobuyuki Yanagihara, Ph.D., Susumu Ueno, M.D., Ph.D., Yuka Sudo, Ph.D., Yasuhito Uezono, M.D., Ph.D., Dan Okura, M.D., Tomoko Minami, M.D., Takashi Kawasaki, M.D., Ph.D., Takeyoshi Sata, M.D., Ph.D. ABSTRACT Background: The neurosteroids allopregnanolone and pregnanolone are potent positive modulators of γ-aminobutyric acid type A receptors. Antinociceptive effects of allopregnanolone have attracted much attention because recent reports have indi- cated the potential of allopregnanolone as a therapeutic agent for refractory pain. However, the analgesic mechanisms of allo- pregnanolone are still unclear. Voltage-gated sodium channels (Na ) are thought to play important roles in inflammatory and neuropathic pain, but there have been few investigations on the effects of allopregnanolone on sodium channels. Methods: Using voltage-clamp techniques, the effects of allopregnanolone sulfate (APAS) and pregnanolone sulfate (PAS) on sodium current were examined in Xenopus oocytes expressing Na 1.2, Na 1.6, Na 1.7, and Na 1.8 α subunits. v v v v Results: APAS suppressed sodium currents of Na 1.2, Na 1.6, and

Journal

Anesthesiology – Wolters Kluwer Health

Published: Sep 1, 2014

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