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Association between GTPase activators for Rho and Ras families

Association between GTPase activators for Rho and Ras families THE ras-related low-molecular-mass GTPases participate in signal transduction involving a variety of cellular functions, including cell-cycle progression, cellular differentiation, cytoskeletal organization, protein transport and secretion1,2. The cycling of these proteins between GTP-bound and GDP-bound states is partially controlled by GTPase activating proteins (GAPs) which stimulate the intrinsic GTP-hydrolysing activity of specific GTPases1–6. The ras GTPase-activating protein (Ras-GAP) forms a complex with a second protein, p190 (M r 190,000), in growth-factor stimulated and tyrosine-kinase transformed cells7,8. At its carboxy-terminal end, p190 contains a region that is conserved in the breakpoint cluster region, n-chimaerin, and Rho-GAP9. Each of these three proteins exhibits GAP activity for at least one member of the rho family of small GTPases10. We have tested recombinant p190 protein for GAP activity on GTPases of the ras, rho and rab families, and show here that p190 can function as a GAP specifically for members of the rho family. Consequently, the formation of a complex between Ras-GAP and p190 in growth-factor stimulated cells may allow the coupling of signalling pathways that involve ras and rho GTPases. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Springer Journals

Association between GTPase activators for Rho and Ras families

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References (30)

Publisher
Springer Journals
Copyright
Copyright © 1992 by Nature Publishing Group
Subject
Science, Humanities and Social Sciences, multidisciplinary; Science, Humanities and Social Sciences, multidisciplinary; Science, multidisciplinary
ISSN
0028-0836
eISSN
1476-4687
DOI
10.1038/359153a0
Publisher site
See Article on Publisher Site

Abstract

THE ras-related low-molecular-mass GTPases participate in signal transduction involving a variety of cellular functions, including cell-cycle progression, cellular differentiation, cytoskeletal organization, protein transport and secretion1,2. The cycling of these proteins between GTP-bound and GDP-bound states is partially controlled by GTPase activating proteins (GAPs) which stimulate the intrinsic GTP-hydrolysing activity of specific GTPases1–6. The ras GTPase-activating protein (Ras-GAP) forms a complex with a second protein, p190 (M r 190,000), in growth-factor stimulated and tyrosine-kinase transformed cells7,8. At its carboxy-terminal end, p190 contains a region that is conserved in the breakpoint cluster region, n-chimaerin, and Rho-GAP9. Each of these three proteins exhibits GAP activity for at least one member of the rho family of small GTPases10. We have tested recombinant p190 protein for GAP activity on GTPases of the ras, rho and rab families, and show here that p190 can function as a GAP specifically for members of the rho family. Consequently, the formation of a complex between Ras-GAP and p190 in growth-factor stimulated cells may allow the coupling of signalling pathways that involve ras and rho GTPases.

Journal

Nature – Springer Journals

Published: Sep 10, 1992

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