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/lp/karger/chicken-embryonic-stem-cells-and-transgenic-strategies-7o1SZQcrH2
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- Publisher
- Karger
- Copyright
- © 1999 S. Karger AG, Basel
- ISSN
- 1422-6405
- eISSN
- 1422-6421
- DOI
- 10.1159/000016701
- Publisher site
- See Article on Publisher Site
Abstract
The production of transgenic birds is an important goal for both fundamental and applied biology. Different methods have been employed to generate transgenic chickens, including microinjection, use of retroviruses and transfection of primordial germ or embryonic germ cells. In this review we will briefly describe these techniques and our efforts to obtain genetically modified avian embryonic stem (ES) cells using liposomes. This latter technique should allow us to modify chicken ES cells with a high efficiency, permitting the rapid generation of transgenic bird lines.
Journal
Cells Tissues Organs – Karger
Published: Jan 1, 1999
Keywords: Transfection; GFP protein; Transgenesis; Embryonic stem cells, chicken