Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 7-Day Trial for You or Your Team.

Learn More →

The power of real-time PCR

The power of real-time PCR In recent years, real-time polymerase chain reaction (PCR) has emerged as a robust and widely used methodology for biological investigation because it can detect and quantify very small amounts of specific nucleic acid sequences. As a research tool, a major application of this technology is the rapid and accurate assessment of changes in gene expression as a result of physiology, pathophysiology, or development. This method can be applied to model systems to measure responses to experimental stimuli and to gain insight into potential changes in protein level and function. Thus physiology can be correlated with molecular events to gain a better understanding of biological processes. For clinical molecular diagnostics, real-time PCR can be used to measure viral or bacterial loads or evaluate cancer status. Here, we discuss the basic concepts, chemistries, and instrumentation of real-time PCR and include present applications and future perspectives for this technology in biomedical sciences and in life science education. Key words: quantitative real-time polymerase chain reaction; method; review; adiponectin receptor http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Advances in Physiology Education The American Physiological Society

The power of real-time PCR

Valasek, Mark A.; Repa, Joyce J.
Advances in Physiology Education , Volume 29 (3): 151 – Sep 1, 2005
Download PDF
10 pages

Loading next page...
 
/lp/the-american-physiological-society/the-power-of-real-time-pcr-4ipCNftDsj

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
The American Physiological Society
Copyright
Copyright © 2010 the American Physiological Society
ISSN
1043-4046
eISSN
1522-1229
DOI
10.1152/advan.00019.2005
pmid
16109794
Publisher site
See Article on Publisher Site

Abstract

In recent years, real-time polymerase chain reaction (PCR) has emerged as a robust and widely used methodology for biological investigation because it can detect and quantify very small amounts of specific nucleic acid sequences. As a research tool, a major application of this technology is the rapid and accurate assessment of changes in gene expression as a result of physiology, pathophysiology, or development. This method can be applied to model systems to measure responses to experimental stimuli and to gain insight into potential changes in protein level and function. Thus physiology can be correlated with molecular events to gain a better understanding of biological processes. For clinical molecular diagnostics, real-time PCR can be used to measure viral or bacterial loads or evaluate cancer status. Here, we discuss the basic concepts, chemistries, and instrumentation of real-time PCR and include present applications and future perspectives for this technology in biomedical sciences and in life science education. Key words: quantitative real-time polymerase chain reaction; method; review; adiponectin receptor

Journal

Advances in Physiology Education – The American Physiological Society

Published: Sep 1, 2005

There are no references for this article.