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References (33)
-
B. Blagoev, Shao-En Ong, I. Kratchmarova, M. Mann (2004)
Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomicsNature Biotechnology, 22
-
Andrea Schlosser, R. Pipkorn, and Bossemeyer, W. Lehmann (2001)
Analysis of protein phosphorylation by a combination of elastase digestion and neutral loss tandem mass spectrometry.Analytical chemistry, 73 2
-
L. Andersson, J. Porath (1986)
Isolation of phosphoproteins by immobilized metal (Fe3+) affinity chromatography.Analytical biochemistry, 154 1
-
J. Rush, A. Moritz, Kimberly Lee, A. Guo, V. Goss, E. Spek, Hui Zhang, Xiangming Zha, R. Polakiewicz, M. Comb (2005)
Immunoaffinity profiling of tyrosine phosphorylation in cancer cellsNature Biotechnology, 23
-
M. Larsen, M. Graham, P. Robinson, P. Roepstorff (2004)
Improved Detection of Hydrophilic Phosphopeptides Using Graphite Powder Microcolumns and Mass SpectrometryMolecular & Cellular Proteomics, 3
-
S. Li, C. Dass (1999)
Iron(III)-immobilized metal ion affinity chromatography and mass spectrometry for the purification and characterization of synthetic phosphopeptides.Analytical biochemistry, 270 1
-
Daniel Schwartz, S. Gygi (2005)
An iterative statistical approach to the identification of protein phosphorylation motifs from large-scale data setsNature Biotechnology, 23
-
T. Nühse, A. Stensballe, O. Jensen, S. Peck (2003)
Large-scale Analysis of in Vivo Phosphorylated Membrane Proteins by Immobilized Metal Ion Affinity Chromatography and Mass Spectrometry*Molecular & Cellular Proteomics, 2
-
Z. Knight, B. Schilling, Richard Row, Denise Kenski, B. Gibson, K. Shokat (2003)
Phosphospecific proteolysis for mapping sites of protein phosphorylationNature Biotechnology, 21
-
J. Garavelli (2004)
The RESID Database of Protein Modifications as a resource and annotation toolPROTEOMICS, 4
-
A. Gruhler, J. Olsen, S. Mohammed, P. Mortensen, N. Færgeman, M. Mann, O. Jensen (2005)
Quantitative Phosphoproteomics Applied to the Yeast Pheromone Signaling Pathway*SMolecular & Cellular Proteomics, 4
-
Joshua Elias, W. Haas, Brendan Faherty, S. Gygi (2005)
Comparative evaluation of mass spectrometry platforms used in large-scale proteomics investigationsNature Methods, 2
-
M. Mann, Shao-En Ong, M. Grønborg, H. Steen, O. Jensen, A. Pandey (2002)
Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome.Trends in biotechnology, 20 6
-
W. Tao, B. Wollscheid, R. O’Brien, J. Eng, Xiao-Jun Li, B. Bodenmiller, J. Watts, L. Hood, R. Aebersold (2005)
Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometryNature Methods, 2
-
MR Larsen, ME Graham, PJ Robinson, P Roepstorff (2004)
Improved detection of hydrophilic phosphopeptides using graphite powder microcolumns and mass spectrometry: evidence for in vivo doubly phosphorylated dynamin I and dynamin IIIMol. Cell. Proteomics, 3
-
R. Aebersold, D. Goodlett (2001)
Mass spectrometry in proteomics.Chemical reviews, 101 2
-
Martijn Pinkse, Pauliina Uitto, M. Hilhorst, B. Ooms, A. Heck (2004)
Selective isolation at the femtomole level of phosphopeptides from proteolytic digests using 2D-NanoLC-ESI-MS/MS and titanium oxide precolumns.Analytical chemistry, 76 14
-
M. Larsen, T. Thingholm, O. Jensen, P. Roepstorff, T. Jørgensen (2005)
Highly Selective Enrichment of Phosphorylated Peptides from Peptide Mixtures Using Titanium Dioxide Microcolumns*Molecular & Cellular Proteomics, 4
-
Andrew Keller, A. Nesvizhskii, E. Kolker, R. Aebersold (2002)
Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search.Analytical chemistry, 74 20
-
Huilin Zhou, J. Watts, R. Aebersold (2001)
A systematic approach to the analysis of protein phosphorylationNature Biotechnology, 19
-
E. Sachon, S. Mohammed, N. Bache, O. Jensen (2006)
Phosphopeptide quantitation using amine-reactive isobaric tagging reagents and tandem mass spectrometry: application to proteins isolated by gel electrophoresis.Rapid communications in mass spectrometry : RCM, 20 7
-
Y. Oda, T. Nagasu, B. Chait (2001)
Enrichment analysis of phosphorylated proteins as a tool for probing the phosphoproteomeNature Biotechnology, 19
-
B. Bodenmiller, Lukas Mueller, Patrick Pedrioli, Delphine Pflieger, Martin Jünger, J. Eng, Ruedi Aebersold, Ruedi Aebersold, W. Tao (2007)
An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells.Molecular bioSystems, 3 4
-
S. Ficarro, M. McCleland, P. Stukenberg, D. Burke, M. Ross, J. Shabanowitz, D. Hunt, F. White (2002)
Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiaeNature Biotechnology, 20
-
Michael Goshe, T. Conrads, E. Panisko, Nicolas Angell, T. Veenstra, Richard Smith (2001)
Phosphoprotein isotope-coded affinity tag approach for isolating and quantitating phosphopeptides in proteome-wide analyses.Analytical chemistry, 73 11
-
M. Gold, T. Yungwirth, Claire Sutherland, R. Ingham, Daisy Vianzon, Readman Chiu, I. Oostveen, H. Morrison, R. Aebersold (1994)
Purification and identification of tyrosine‐phosphorylated proteins from B lymphocytes stimulated through the antigen receptorELECTROPHORESIS, 15
-
J. Reinders, A. Sickmann (2005)
State‐of‐the‐art in phosphoproteomicsPROTEOMICS, 5
-
Y. Kanakura, B. Druker, J. DiCarlo, S. Cannistra, J. Griffin (1991)
Phorbol 12-myristate 13-acetate inhibits granulocyte-macrophage colony stimulating factor-induced protein tyrosine phosphorylation in a human factor-dependent hematopoietic cell line.The Journal of biological chemistry, 266 1
-
(2000)
NATURE METHODS ARTICLES © 20 07 N at ur e P ub lis hi ng G ro up ht tp :// w w w .n at ur e. co m /n at ur em et h o d s -
M. Posewitz, P. Tempst (1999)
Immobilized gallium(III) affinity chromatography of phosphopeptides.Analytical chemistry, 71 14
-
Hye Kweon, K. Håkansson (2006)
Selective zirconium dioxide-based enrichment of phosphorylated peptides for mass spectrometric analysis.Analytical chemistry, 78 6
-
P. Cao, J. Stults (1999)
Phosphopeptide analysis by on-line immobilized metal-ion affinity chromatography-capillary electrophoresis-electrospray ionization mass spectrometry.Journal of chromatography. A, 853 1-2
-
T. Hunter (2000)
Signaling—2000 and BeyondCell, 100
- Publisher
- Springer Journals
- Copyright
- Copyright © 2007 by Nature Publishing Group
- Subject
- Life Sciences; Life Sciences, general; Biological Techniques; Biological Microscopy; Biomedical Engineering/Biotechnology; Bioinformatics; Proteomics
- ISSN
- 1548-7091
- eISSN
- 1548-7105
- DOI
- 10.1038/nmeth1005
- Publisher site
- See Article on Publisher Site
Abstract
The ability to routinely analyze and quantitatively measure changes in protein phosphorylation on a proteome-wide scale is essential for biological and clinical research. We assessed the ability of three common phosphopeptide isolation methods (phosphoramidate chemistry (PAC), immobilized metal affinity chromatography (IMAC) and titanium dioxide) to reproducibly, specifically and comprehensively isolate phosphopeptides from complex mixtures. Phosphopeptides were isolated from aliquots of a tryptic digest of the cytosolic fraction of Drosophila melanogaster Kc167 cells and analyzed by liquid chromatography–electrospray ionization tandem mass spectrometry. Each method reproducibly isolated phosphopeptides. The methods, however, differed in their specificity of isolation and, notably, in the set of phosphopeptides isolated. The results suggest that the three methods detect different, partially overlapping segments of the phosphoproteome and that, at present, no single method is sufficient for a comprehensive phosphoproteome analysis.
Journal
Nature Methods – Springer Journals
Published: Feb 11, 2007