Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/springer-journals/enzymes-in-nonaqueous-solvents-the-effects-of-crown-ethers-on-the-2KoIAXGlog
References (10)
-
Katherine Debulis, A. Klibanov (1993)
Dramatic enhancement of enzymatic activity in organic solvents by lyoprotectantsBiotechnology and Bioengineering, 41
-
J. Dordick (1989)
Enzymatic catalysis in monophasic organic solventsEnzyme and Microbial Technology, 11
-
J. Engbersen, J. Broos, W. Verboom, D. Reinhoudt (1996)
Effects of crown ethers and small amounts of cosolvent on the activity and enantioselectivity of α-chymotrypsin in organic sofventsPure and Applied Chemistry, 68
-
Zu‐Feng Xu, R. Affleck, P. Wangikar, V. Suzawa, J. Dordick, D. Clark (1994)
Transition state stabilization of subtilisins in organic mediaBiotechnology and Bioengineering, 43
-
van DJ, Engbersen, Reinhoudt (1998)
Large acceleration of alpha-chymotrypsin-catalyzed dipeptide formation by 18-crown-6 in organic solventsBiotechnology and bioengineering, 59 5
-
J. Schmitke, C. Wescott, A. Klibanov (1996)
THE MECHANISTIC DISSECTION OF THE PLUNGE IN ENZYMATIC ACTIVITY UPON TRANSITION FROM WATER TO ANHYDROUS SOLVENTSJournal of the American Chemical Society, 118
-
P. Halling (1992)
Salt hydrates for water activity control with biocatalysts in organic mediaBiotechnology Techniques, 6
-
González-Navarro, Braco (1998)
Lipase-enhanced activity in flavour ester reactions by trapping enzyme conformers in the presence of interfacesBiotechnology and bioengineering, 59 1
-
M. Otamiri, P. Adlercreutz, B. Mattiasson (1992)
Complex Formation Between Chymotrypsin and Ethyl Cellulose as a Means to Solubilize the Enzyme in Active Form in TolueneBiocatalysis and Biotransformation, 6
-
C. Pedersen (1967)
Cyclic polyethers and their complexes with metal saltsJournal of the American Chemical Society, 89
- Publisher
- Humana Press
- Copyright
- © Humana Press 2001
- ISBN
- 978-0-89603-929-2
- Pages
- 213–225
- DOI
- 10.1385/1-59259-112-4:213
- Publisher site
- See Chapter on Publisher Site
Abstract
[Currently, the applicability of enzymes in synthetic organic chemistry is well recognized. The field of enzyme-catalyzed organic synthesis has been further boosted by the recognition that enzymes can operate in organic solvents. The use of nonaqueous media for enzymatic conversions offers a number of advantages, like enhanced thermal stability of the enzyme, increased substrate solubility, a shift of the equilibrium in favor of synthesis over hydrolysis, and altered selectivity properties of the enzyme (1). However, the most important drawback of the use of enzymes in organic media is the reduced catalytic activity compared to aqueous conditions. Typically, this reduction in activity is two to six orders of magnitude (2). However, studies in our laboratory have revealed that (pre)treatment of the enzymes with crown ethers can enhance enzyme activities in nonaqueous organic solvents up to a level approaching the activity under aqueous conditions.]
Published: Jan 1, 2001
Keywords: Crown Ether; Organic Medium; Vinyl Acetate; Amino Acid Ester; Nonaqueous Medium