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A Human Melanoma Line Heterogeneous With Respect to Metastatic Capacity in Athymic Nude Mice

A Human Melanoma Line Heterogeneous With Respect to Metastatic Capacity in Athymic Nude Mice Abstract The ability of the human A375 melanoma cell line to produce experimental and spontaneous metastases in young BALB/c nude mice was examined. Cloned lines, obtained by isolation in semisolid agar, differed significantly (4/10 cloned lines examined, P≤.005) from the parent tumor line with regard to their ability to form lung tumor nodules subsequent to iv injection. Lines established from such lung tumor nodules were more metastatic than the parent line following both iv and sc injection. These results show that the human melanoma cell line used in these studies contained cells with diverse metastatic potential and suggest that metastasis in the nude mouse results from the preferential selection of metastatic subpopulations. 2 Research sponsored by the National Cancer Institute, U.S. Department of Health and Human Services, under contract N01CO-23909 with Litton Bionetics, Inc. 3 The contents of this publication do not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government. 4 Animals were maintained under the guidelines set forth by the National Institutes of Health Policy on Humane Care and Use of Animals and by the Animal Welfare Act and in facilities recognized by the American Association for Accreditation of Laboratory Animal Care. This content is only available as a PDF. Author notes 5 Cancer Metastasis and Treatment Laboratory, LBI-Basic Research Program, NCI-Frederick Cancer Research Facility, P.O. Box B, Frederick, Maryland 21701. 6 A Burroughs Wellcome Scholar of the American Urological Association and a Leander Riba Fellow of Northwestern University Medical School. 8 Smith Kline and French Laboratories, 620 Allendale Rd., King of Prussia, Pa. 19406. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png JNCI: Journal of the National Cancer Institute Oxford University Press

A Human Melanoma Line Heterogeneous With Respect to Metastatic Capacity in Athymic Nude Mice

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Publisher
Oxford University Press
ISSN
0027-8874
eISSN
1460-2105
DOI
10.1093/jnci/72.4.913
Publisher site
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Abstract

Abstract The ability of the human A375 melanoma cell line to produce experimental and spontaneous metastases in young BALB/c nude mice was examined. Cloned lines, obtained by isolation in semisolid agar, differed significantly (4/10 cloned lines examined, P≤.005) from the parent tumor line with regard to their ability to form lung tumor nodules subsequent to iv injection. Lines established from such lung tumor nodules were more metastatic than the parent line following both iv and sc injection. These results show that the human melanoma cell line used in these studies contained cells with diverse metastatic potential and suggest that metastasis in the nude mouse results from the preferential selection of metastatic subpopulations. 2 Research sponsored by the National Cancer Institute, U.S. Department of Health and Human Services, under contract N01CO-23909 with Litton Bionetics, Inc. 3 The contents of this publication do not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government. 4 Animals were maintained under the guidelines set forth by the National Institutes of Health Policy on Humane Care and Use of Animals and by the Animal Welfare Act and in facilities recognized by the American Association for Accreditation of Laboratory Animal Care. This content is only available as a PDF. Author notes 5 Cancer Metastasis and Treatment Laboratory, LBI-Basic Research Program, NCI-Frederick Cancer Research Facility, P.O. Box B, Frederick, Maryland 21701. 6 A Burroughs Wellcome Scholar of the American Urological Association and a Leander Riba Fellow of Northwestern University Medical School. 8 Smith Kline and French Laboratories, 620 Allendale Rd., King of Prussia, Pa. 19406.

Journal

JNCI: Journal of the National Cancer Institute – Oxford University Press

Published: Apr 1, 1984

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